BRENDA - Enzyme Database show
show all sequences of 1.14.14.45

CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis

Naur, P.; Petersen, B.L.; Mikkelsen, M.D.; Bak, S.; Rasmussen, H.; Olsen, C.E.; Halkier, B.A.; Plant Physiol. 133, 63-72 (2003)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.065
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.188
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O65782
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
additional information
CYP83B1 metabolizes aliphatic oximes with very low efficiency
741196
Arabidopsis thaliana
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.23
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.78
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.87
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.065
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.188
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
741196
Arabidopsis thaliana
S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O
overall reaction
-
-
?
additional information
CYP83B1 metabolizes aliphatic oximes with very low efficiency
741196
Arabidopsis thaliana
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.23
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.78
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
0.87
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
physiological function
a CYP83B1 knockout mutant, rnt1-1, leads to the the presence of indole glucosinolates at various levels in different developmental stages. Plants overexpressing CYP83B1 contain elevated levels of aliphatic glucosinolates derived from methionine homologs, whereas the level of indole glucosinolates is almost constant in the overexpressing lines
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
a CYP83B1 knockout mutant, rnt1-1, leads to the the presence of indole glucosinolates at various levels in different developmental stages. Plants overexpressing CYP83B1 contain elevated levels of aliphatic glucosinolates derived from methionine homologs, whereas the level of indole glucosinolates is almost constant in the overexpressing lines
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.7
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
4.2
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
267
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.7
-
(E)-p-hydroxyphenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
4.2
-
(E)-phenylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
267
-
(E)-indol-3-ylacetaldehyde oxime
pH 8.1, 29°C
Arabidopsis thaliana
Other publictions for EC 1.14.14.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739318
Clausen
The bifurcation of the cyanoge ...
Arabidopsis thaliana
Plant J.
84
558-573
2015
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2
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741163
Kim
Indole glucosinolate biosynthe ...
Arabidopsis thaliana
Plant Cell
27
1529-1546
2015
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1
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1
1
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743473
Clausen
The bifurcation of the cyanog ...
Arabidopsis thaliana
Plant J.
84
558-573
2015
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4
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1
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4
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740546
Zhu
Isolation and expression of gl ...
Brassica rapa subsp. chinensis
Int. J. Mol. Sci.
13
5832-5843
2012
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1
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3
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3
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1
1
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741152
Geu-Flores
Cytosolic gamma-glutamyl pepti ...
Arabidopsis thaliana
Plant Cell
23
2456-2469
2011
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1
1
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741196
Naur
CYP83A1 and CYP83B1, two nonre ...
Arabidopsis thaliana
Plant Physiol.
133
63-72
2003
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3
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1
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4
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4
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3
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1
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3
3
740514
Smolen
Arabidopsis cytochrome P450 cy ...
Arabidopsis thaliana
Genetics
160
323-332
2002
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1
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740669
Hansen
CYP83B1 is the oxime-metaboliz ...
Arabidopsis thaliana
J. Biol. Chem.
276
24790-24796
2001
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1
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1
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1
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1
1
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1
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1
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2
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1
1
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741148
Bak
CYP83B1, a cytochrome P450 at ...
Arabidopsis thaliana
Plant Cell
13
101-111
2001
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1
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1
1
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1
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1
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1
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1
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1
1
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741195
Bak
The involvement of two p450 en ...
Arabidopsis thaliana
Plant Physiol.
127
108-118
2001
-
-
-
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1
1
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3
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1
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3
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3
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3
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3
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3
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1
1
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743440
Bak
CYP83B1, a cytochrome P450 at ...
Arabidopsis thaliana
Plant Cell
13
101-111
2001
-
-
-
-
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-
1
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
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743483
Bak
The involvement of two p450 e ...
Arabidopsis thaliana
Plant Physiol.
127
108-118
2001
-
-
1
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1
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5
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1
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5
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1
1
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