Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Bacillus subtilis |
45348 | - |
x * 45348, electrospray ionization mass spectrometry | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 | Bacillus subtilis | - |
pimeloyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-300 gel filtration | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (7R,8R)-7,8-dihydroxy-long-chain-acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a 7-oxoheptanoyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + 2 H2O | 1c | Bacillus subtilis | |
a (7S)-7-hydroxy-long-chain-acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a (7R,8R)-7,8-dihydroxy-long-chain-acyl-[acyl-carrier protein] + oxidized flavodoxin + H2O | 1b | Bacillus subtilis | |
a 7-oxoheptanoyl-[acyl-carrier protein] + oxidized flavodoxin + H2O = a pimeloyl-[acyl-carrier protein] + reduced flavodoxin + H+ | 1d | Bacillus subtilis | |
a long-chain acyl-[acyl-carrier protein] + 2 reduced flavodoxin + 3 O2 = pimeloyl-[acyl-carrier protein] + an n-alkanal + 2 oxidized flavodoxin + 3 H2O | overall reaction | Bacillus subtilis | |
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a (7S)-7-hydroxy-long-chain-acyl-[acyl-carrier protein] + oxidized flavodoxin + H2O | 1a | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 | - |
Bacillus subtilis | pimeloyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + H2O | - |
r | |
myristoyl-[acyl-carrier protein] + reduced flavodoxin + O2 | - |
Bacillus subtilis | ? | - |
r | |
oleoyl-[acyl-carrier protein] + reduced flavodoxin + O2 | - |
Bacillus subtilis | ? | - |
r | |
palmitoleoyl-[acyl-carrier protein] + reduced flavodoxin + O2 | - |
Bacillus subtilis | ? | - |
r | |
palmitoyl-[acyl-carrier protein] + reduced flavodoxin + O2 | - |
Bacillus subtilis | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Bacillus subtilis |
? | x * 45348, electrospray ionization mass spectrometry | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
CYP107H1 | - |
Bacillus subtilis |
P450BioI | - |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | calculated from amino acid sequence | - |
6.9 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in biotin biosynthesis | Bacillus subtilis |