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Literature summary for 1.14.14.46 extracted from

  • Stok, J.E.; De Voss, J.
    Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis (2000), Arch. Biochem. Biophys., 384, 351-360.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Bacillus subtilis
45348
-
x * 45348, electrospray ionization mass spectrometry Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 Bacillus subtilis
-
pimeloyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-300 gel filtration Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
a (7R,8R)-7,8-dihydroxy-long-chain-acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a 7-oxoheptanoyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + 2 H2O 1c Bacillus subtilis
a (7S)-7-hydroxy-long-chain-acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a (7R,8R)-7,8-dihydroxy-long-chain-acyl-[acyl-carrier protein] + oxidized flavodoxin + H2O 1b Bacillus subtilis
a 7-oxoheptanoyl-[acyl-carrier protein] + oxidized flavodoxin + H2O = a pimeloyl-[acyl-carrier protein] + reduced flavodoxin + H+ 1d Bacillus subtilis
a long-chain acyl-[acyl-carrier protein] + 2 reduced flavodoxin + 3 O2 = pimeloyl-[acyl-carrier protein] + an n-alkanal + 2 oxidized flavodoxin + 3 H2O overall reaction Bacillus subtilis
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = a (7S)-7-hydroxy-long-chain-acyl-[acyl-carrier protein] + oxidized flavodoxin + H2O 1a Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2
-
Bacillus subtilis pimeloyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + H2O
-
r
myristoyl-[acyl-carrier protein] + reduced flavodoxin + O2
-
Bacillus subtilis ?
-
r
oleoyl-[acyl-carrier protein] + reduced flavodoxin + O2
-
Bacillus subtilis ?
-
r
palmitoleoyl-[acyl-carrier protein] + reduced flavodoxin + O2
-
Bacillus subtilis ?
-
r
palmitoyl-[acyl-carrier protein] + reduced flavodoxin + O2
-
Bacillus subtilis ?
-
r

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Bacillus subtilis
? x * 45348, electrospray ionization mass spectrometry Bacillus subtilis

Synonyms

Synonyms Comment Organism
CYP107H1
-
Bacillus subtilis
P450BioI
-
Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis calculated from amino acid sequence
-
6.9

General Information

General Information Comment Organism
metabolism the enzyme is involved in biotin biosynthesis Bacillus subtilis