Crystallization (Comment) | Organism |
---|---|
enzyme exhibits two conformations in the absence of substrate. The addition of L-Arg stabilizes the conformer more similar to the Mus muculus enzyme, whereas N-hydroxy-L-arginine stabilizes the conformer more similar to the Bacillus subtilis enzyme, although both substrates introduce a positive electrostatic potential to the distal heme pocket | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | in the substrate-free form, the Fe-Cys stretching mode is detected at 342.5 per cm, similar to that of Bacillus subtilis NOS. The binding of L-Arg and N-hydroxy-L-arginine brings about a small decrease and increase in the Fe-Cys stretching frequency, respectively | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |