Application | Comment | Organism |
---|---|---|
analysis | development of a robust enzyme assay for measuring NOS activity and inhibition | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
wild-type in complex with inhibitors N-omega-nitro-L-arginine and 3-bromo-7-nitroindazole | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a chimera between enzyme and flavodoxin YkuN is 8fold more active tan wild-type. Adding excess amounts of YkuN does not significantly alter activity | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-bromo-7-nitroindazole | - |
Bacillus subtilis | |
7-nitroindazole | - |
Bacillus subtilis | |
N-omega-nitro-L-arginine | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34453 | - |
- |
Bacillus subtilis 168 | O34453 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 reduced flavodoxin + 4 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O | overall reaction | ? | |
2 L-arginine + 3 reduced flavodoxin + 4 O2 | - |
Bacillus subtilis 168 | 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O | overall reaction | ? | |
additional information | NO production requires flavodoxin YkuN and a flavodoxin reductase, YumC fulfilling this requirement | Bacillus subtilis | ? | - |
? | |
additional information | NO production requires flavodoxin YkuN and a flavodoxin reductase, YumC fulfilling this requirement | Bacillus subtilis 168 | ? | - |
? | |
N-omega-hydroxy-L-arginine + reduced flavodoxin + 2 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + oxidized flavodoxin + 2 H2O | - |
? | |
N-omega-hydroxy-L-arginine + reduced flavodoxin + 2 O2 | - |
Bacillus subtilis 168 | 2 L-citrulline + 2 nitric oxide + oxidized flavodoxin + 2 H2O | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0027 | - |
chimera between enzyme and flavodoxin YkuN, pH 7.4, 35°C | Bacillus subtilis | 7-nitroindazole | |
0.0102 | - |
chimera between enzyme and flavodoxin YkuN, pH 7.4, 35°C | Bacillus subtilis | N-omega-nitro-L-arginine |