BRENDA - Enzyme Database show
show all sequences of 1.14.14.48

CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite

Kitaoka, N.; Kawaide, H.; Amano, N.; Matsubara, T.; Nabeta, K.; Takahashi, K.; Matsuura, H.; Phytochemistry 99, 6-13 (2014)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a jasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9SMP5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a jasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-leucine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-leucine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-phenylalanine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-phenylalanine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-valine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-valine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with jasmonoyl-L-alanine, jasmonoyl-glycine, jasmonoyl-L-tryptophan, and jasmonoyl-1-aminocyclopropane-1-carboxylic acid
743433
Arabidopsis thaliana
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a jasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a jasmonoyl-L-amino acid + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-leucine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-leucine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-phenylalanine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-phenylalanine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
jasmonoyl-L-valine + [reduced NADPH-hemoprotein reductase] + O2
-
743433
Arabidopsis thaliana
12-hydroxyjasmonoyl-L-valine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with jasmonoyl-L-alanine, jasmonoyl-glycine, jasmonoyl-L-tryptophan, and jasmonoyl-1-aminocyclopropane-1-carboxylic acid
743433
Arabidopsis thaliana
?
-
-
-
-
Other publictions for EC 1.14.14.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741992
Poudel
Mutations in jasmonoyl-L-isol ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1861
1396-1408
2016
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6
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1
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2
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1
2
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742727
Luo
COI1-regulated hydroxylation ...
Nicotiana attenuata
J. Agric. Food Chem.
64
2822-2831
2016
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6
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1
1
1
1
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743607
Bruckhoff
Functional characterization o ...
Arabidopsis thaliana
PLoS ONE
11
e0159875
2016
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4
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4
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2
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8
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2
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1
2
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742999
Aubert
CYP94-mediated jasmonoyl-isol ...
Arabidopsis thaliana
J. Exp. Bot.
66
3879-3892
2015
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4
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1
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1
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1
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1
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1
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1
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743430
Widemann
Sequential oxidation of jasmo ...
Arabidopsis thaliana
Phytochemistry
117
388-399
2015
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1
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3
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2
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1
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2
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742863
Koo
Endoplasmic reticulum-associa ...
Arabidopsis thaliana
J. Biol. Chem.
289
29728-29738
2014
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1
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2
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4
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1
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2
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2
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2
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2
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2
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743433
Kitaoka
CYP94B3 activity against jasm ...
Arabidopsis thaliana
Phytochemistry
99
6-13
2014
-
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-
-
-
-
-
-
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1
-
1
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6
-
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1
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6
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742840
Heitz
Cytochromes P450 CYP94C1 and ...
Arabidopsis thaliana
J. Biol. Chem.
287
6296-6306
2012
-
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1
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-
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1
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4
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1
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1
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1
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1
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1
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1
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743453
Kitaoka
Arabidopsis CYP94B3 encodes j ...
Arabidopsis thaliana
Plant Cell Physiol.
52
1757-1765
2011
-
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1
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4
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1
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1
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1
1
1
1
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743675
Koo
Cytochrome P450 CYP94B3 media ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
108
9298-9303
2011
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1
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3
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1
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