BRENDA - Enzyme Database show
show all sequences of 1.14.14.51

Cloning and expression of the limonene hydroxylase of Bacillus stearothermophilus BR388 and utilization in two-phase limonene conversions

Cheong, T.; Oriel, P.; Appl. Biochem. Biotechnol. 84-86, 903-915 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Geobacillus stearothermophilus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
Geobacillus stearothermophilus
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
Geobacillus stearothermophilus BR388
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Geobacillus stearothermophilus
O85057
-
-
Geobacillus stearothermophilus BR388
O85057
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
741662
Geobacillus stearothermophilus
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
741662
Geobacillus stearothermophilus BR388
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
-
?
3-cyclohexene 1-methanol + NAD+
9.6% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
3-cyclohexenylaldehyde + NADH
-
-
-
?
3-cyclohexene 1-methanol + NAD+
9.6% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
3-cyclohexenylaldehyde + NADH
-
-
-
?
4-isopropyl benzyl alcohol + NAD+
42.2% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
4-isopropylbenzyl aldehyde + NADH
-
-
-
?
4-isopropyl benzyl alcohol + NAD+
42.2% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
4-isopropylbenzyl aldehyde + NADH
-
-
-
?
4-mrthyl benzyl alcohol + NAD+
17.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
? + NADH
-
-
-
?
benzyl alcohol + NAD+
3.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
benzaldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
3.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
benzaldehyde + NADH
-
-
-
?
carveol + NAD+
86% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
? + NADH
-
-
-
?
cyclohexyl methanol + NAD+
15.8% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
cyclohexylaldehyde + NADH
-
-
-
?
myrtenol + NAD+
4.0% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
myrtenal + NADH
-
-
-
?
perillyl alcohol + NAD+
100% activity
741662
Geobacillus stearothermophilus
perillyl aldehyde + NADH
-
-
-
r
perillyl alcohol + NAD+
100% activity
741662
Geobacillus stearothermophilus BR388
perillyl aldehyde + NADH
-
-
-
r
perillyl aldehyde + NADH
-
741662
Geobacillus stearothermophilus
perillyl alcohol + NAD+
-
-
-
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Geobacillus stearothermophilus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
55
about 35% activity at 30C, about 55% activity at 37C, about 90% activity at 40C, 100% activity at 45C, about 75% activity at 50C, about 30% activity at 55C
Geobacillus stearothermophilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Geobacillus stearothermophilus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
about 40% activity at pH 5.5, about 75% activity at pH 6.5, about 90% activity at pH 7.0, 100% activity at 8.0, about 75% activity at pH 9.0, about 40% activity at pH 10.0
Geobacillus stearothermophilus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
stimulates the hydroxylation reaction
Geobacillus stearothermophilus
NAD+
stimulates the dehydrogenase reaction
Geobacillus stearothermophilus
NADH
stimulates the hydroxylation reaction
Geobacillus stearothermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Geobacillus stearothermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
stimulates the hydroxylation reaction
Geobacillus stearothermophilus
NAD+
stimulates the dehydrogenase reaction
Geobacillus stearothermophilus
NADH
stimulates the hydroxylation reaction
Geobacillus stearothermophilus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
Geobacillus stearothermophilus
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
Geobacillus stearothermophilus BR388
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
741662
Geobacillus stearothermophilus
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
741662
Geobacillus stearothermophilus BR388
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
-
-
?
3-cyclohexene 1-methanol + NAD+
9.6% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
3-cyclohexenylaldehyde + NADH
-
-
-
?
3-cyclohexene 1-methanol + NAD+
9.6% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
3-cyclohexenylaldehyde + NADH
-
-
-
?
4-isopropyl benzyl alcohol + NAD+
42.2% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
4-isopropylbenzyl aldehyde + NADH
-
-
-
?
4-isopropyl benzyl alcohol + NAD+
42.2% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
4-isopropylbenzyl aldehyde + NADH
-
-
-
?
4-mrthyl benzyl alcohol + NAD+
17.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
? + NADH
-
-
-
?
benzyl alcohol + NAD+
3.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
benzaldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
3.1% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus BR388
benzaldehyde + NADH
-
-
-
?
carveol + NAD+
86% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
? + NADH
-
-
-
?
cyclohexyl methanol + NAD+
15.8% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
cyclohexylaldehyde + NADH
-
-
-
?
myrtenol + NAD+
4.0% activity compared to perillyl alcohol
741662
Geobacillus stearothermophilus
myrtenal + NADH
-
-
-
?
perillyl alcohol + NAD+
100% activity
741662
Geobacillus stearothermophilus
perillyl aldehyde + NADH
-
-
-
r
perillyl alcohol + NAD+
100% activity
741662
Geobacillus stearothermophilus BR388
perillyl aldehyde + NADH
-
-
-
r
perillyl aldehyde + NADH
-
741662
Geobacillus stearothermophilus
perillyl alcohol + NAD+
-
-
-
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Geobacillus stearothermophilus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
55
about 35% activity at 30C, about 55% activity at 37C, about 90% activity at 40C, 100% activity at 45C, about 75% activity at 50C, about 30% activity at 55C
Geobacillus stearothermophilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Geobacillus stearothermophilus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
about 40% activity at pH 5.5, about 75% activity at pH 6.5, about 90% activity at pH 7.0, 100% activity at 8.0, about 75% activity at pH 9.0, about 40% activity at pH 10.0
Geobacillus stearothermophilus
Other publictions for EC 1.14.14.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
660251
Turner
Organization of monoterpene bi ...
Mentha spicata
Plant Physiol.
136
4215-4227
2004
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1
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4
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1
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656954
Carter
Monoterpene biosynthesis pathw ...
Mentha spicata
Phytochemistry
64
425-433
2003
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1
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3
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1
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717888
Hamada
Stereoselective biotransformat ...
Synechococcus sp., Synechococcus sp. PCC 7942
J. Biosci. Bioeng.
96
581-584
2003
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1
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4
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19
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8
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1
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1
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1
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4
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8
-
1
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1
-
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-
390094
Wuest
Hydroxylation of specifically ...
Mentha spicata
Biochemistry
41
1820-1827
2002
-
-
1
-
-
-
-
-
-
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1
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1
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2
-
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658534
Shimada
Species differences in the met ...
Canis lupus familiaris, Cavia porcellus, Homo sapiens, Macaca fascicularis, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Drug Metab. Pharmacokinet.
17
507-515
2002
-
-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
7
-
-
7
-
-
-
-
-
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-
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7
-
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-
7
-
-
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-
7
-
-
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7
-
-
7
-
-
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-
-
-
-
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-
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-
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-
390089
Wust
Hydroxylation of limonene enan ...
Mentha spicata
Arch. Biochem. Biophys.
387
125-136
2001
-
-
-
-
-
-
-
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-
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1
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2
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-
-
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390086
Schalk
A single amino acid substituti ...
Mentha spicata
Proc. Natl. Acad. Sci. USA
97
11948-11953
2000
-
-
-
-
-
-
-
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3
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1
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1
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-
-
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390088
Haudenschild
Functional expression of regio ...
Mentha spicata
Arch. Biochem. Biophys.
379
127-136
2000
-
-
1
-
-
-
-
-
-
-
1
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1
-
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1
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1
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1
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1
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1
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741662
Cheong
Cloning and expression of the ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus BR388
Appl. Biochem. Biotechnol.
84-86
903-915
2000
-
-
1
-
-
-
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-
2
-
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2
-
4
-
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-
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15
-
1
1
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1
1
-
3
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1
3
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2
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2
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-
-
15
-
1
1
-
-
1
1
-
-
-
-
-
-
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-
390087
Lupien
Regiospecific cytochrome P450 ...
Mentha spicata
Arch. Biochem. Biophys.
368
181-192
1999
-
-
1
-
-
-
-
-
1
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1
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3
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1
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1
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1
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743432
Bouwmeester
-
Cytochrome P-450 dependent (+ ...
Carum carvi
Phytochemistry
50
243-248
1999
-
-
-
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-
6
-
1
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1
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1
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1
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1
1
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1
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3
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3
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6
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1
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1
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1
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1
1
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1
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-
390097
Lupien
Cytochrome P450 limonene hydro ...
Mentha spicata
Drug Metabol. Drug Interact.
12
245-260
1995
-
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1
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1
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4
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1
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1
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2
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-
390085
Gershenzon
Isolation of secretory cells f ...
Mentha spicata
Anal. Biochem.
200
130-138
1992
-
-
-
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3
-
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1
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1
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1
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1
1
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1
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1
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-
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3
-
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-
-
1
-
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-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390084
Croteau
Biochemical characterization o ...
Mentha x gracilis
Plant Physiol.
96
744-752
1991
-
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2
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1
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1
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1
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1
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4
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2
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1
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1
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1
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4
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-
390083
Karp
Monoterpene biosynthesis: spec ...
Mentha spicata
Arch. Biochem. Biophys.
276
219-226
1990
-
-
-
-
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-
9
1
1
-
-
1
-
1
-
-
1
-
-
1
1
-
6
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
1
1
-
-
1
-
-
-
1
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286368
Gershenzon
Biochemical and histochemical ...
Mentha spicata
Plant Physiol.
89
1351-1357
1989
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