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Literature summary for 1.14.14.53 extracted from

  • Bouwmeester, H.J.; Konings, M.C.J.M.; Gershenzon, J.; Karp, F.; Croteau, R.
    Cytochrome P-450 dependent (+)-limonene-6-hydroxylation in fruits of caraway (Carum Carvi) (1999), Phytochemistry, 50, 243-248.
No PubMed abstract available
Search for more literature for 1.14.14.53

General Stability

General Stability Organism
polyvinylpyrrolidone, NaHsO3, sodium diethyldithiocarbamate, leupeptin, EGTA, catalase, and glutathione stabilize microsomal extract Carum carvi

Inhibitors

Inhibitors Comment Organism Structure
clotrimazole less than 0.001 mM, 50% inhibition Carum carvi
cytochrome c less than 0.01 mM, 50% inhibition Carum carvi
metapyrone 1.5 mM, 50% inhibition Carum carvi
miconazole 0.048 mM, 50% inhibition Carum carvi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0114
-
 (+)-limonene annual caraway Carum carvi
0.0149
-
 (+)-limonene biannual caraway Carum carvi

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Carum carvi
-
-

Organism

Organism UniProt Comment Textmining
Carum carvi
-
 var Karzo, annual caraway, var Beija, biennial caraway
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Carum carvi
-

Storage Stability

Storage Stability Organism
-80 C, microsomal preparation, 1 year, no loss of activity Carum carvi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-limonene + NADPH + H+ + O2 high regio- and stereospecificity, requiring NADPH Carum carvi (+)-trans-carveol + NADP+ + H2O 97% of total product, 2.5% (-)-trans-carveol, 0.5% (-)-cis-carveol ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Carum carvi

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8.4
-
 Carum carvi

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Carum carvi
NADP+ no activity with NAD+ Carum carvi