BRENDA - Enzyme Database show
show all sequences of 1.14.14.54

Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction

Mingot, J.; Peñalva, M.; Fernandez-Canon, J.; J. Biol. Chem. 274, 14545-14550 (1999)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetate + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus nidulans
-
(2-hydroxyphenyl)acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus nidulans
Q9Y7G5
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus nidulans
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phenylacetate + [reduced NADPH-hemoprotein reductase] + O2
-
742821
Aspergillus nidulans
(2-hydroxyphenyl)acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus nidulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus nidulans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetate + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus nidulans
-
(2-hydroxyphenyl)acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus nidulans
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phenylacetate + [reduced NADPH-hemoprotein reductase] + O2
-
742821
Aspergillus nidulans
(2-hydroxyphenyl)acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Aspergillus nidulans
gene transcription is strongly induced by phenylacetate
up
General Information
General Information
Commentary
Organism
malfunction
enzyme gene disruption results in increased penicillin production
Aspergillus nidulans
General Information (protein specific)
General Information
Commentary
Organism
malfunction
enzyme gene disruption results in increased penicillin production
Aspergillus nidulans
Expression (protein specific)
Organism
Commentary
Expression
Aspergillus nidulans
gene transcription is strongly induced by phenylacetate
up
Other publictions for EC 1.14.14.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742770
Rodriguez-Saiz
Reduced function of a phenyla ...
Penicillium chrysogenum, Penicillium chrysogenum NRRL 1951
J. Bacteriol.
183
5465-5471
2001
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742821
Mingot
Disruption of phacA, an Asper ...
Aspergillus nidulans
J. Biol. Chem.
274
14545-14550
1999
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