BRENDA - Enzyme Database show
show all sequences of 1.14.14.56

Roles of cytochrome P450 3A enzymes in the 2-hydroxylation of 1,4-cineole, a monoterpene cyclic ether, by rat and human liver microsomes

Miyazawa, M.; Shindo, M.; Shimada, T.; Xenobiotica 31, 713-723 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dexamethasone
induces activity
Rattus norvegicus
pregnenolone 16alpha-carbonitrile
induces activity
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
expression in insect cells
Homo sapiens
expression in insect cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ketoconazole
significant inhibition
Homo sapiens
ketoconazole
significant inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.45
-
1,4-cineole
pH 7.4, 37°C
Homo sapiens
1.033
-
1,4-cineole
pH 7.4, 37°C, animals treated with pregnenolone 16alpha-carbonitrile
Rattus norvegicus
1.123
-
1,4-cineole
pH 7.4, 37°C, animals treated with dexamethasone
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Homo sapiens
2-exo-hydroxy-1,4-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,4-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Rattus norvegicus
2-exo-hydroxy-1,4-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Homo sapiens
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Rattus norvegicus
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dexamethasone
induces activity
Rattus norvegicus
pregnenolone 16alpha-carbonitrile
induces activity
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in insect cells
Homo sapiens
expression in insect cells
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ketoconazole
significant inhibition
Homo sapiens
ketoconazole
significant inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.45
-
1,4-cineole
pH 7.4, 37°C
Homo sapiens
1.033
-
1,4-cineole
pH 7.4, 37°C, animals treated with pregnenolone 16alpha-carbonitrile
Rattus norvegicus
1.123
-
1,4-cineole
pH 7.4, 37°C, animals treated with dexamethasone
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Homo sapiens
2-exo-hydroxy-1,4-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,4-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Rattus norvegicus
2-exo-hydroxy-1,4-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Homo sapiens
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
718453
Rattus norvegicus
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717270
Duisken
Metabolism of 1,8-cineole by h ...
Homo sapiens
Biochim. Biophys. Acta
1722
304-311
2005
-
-
1
-
-
-
-
4
1
-
-
-
-
1
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1
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2
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1
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1
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1
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2
-
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-
-
-
-
-
2
2
717531
Miyazawa
Oxidation of 1,8-cineole, the ...
Rattus norvegicus
Drug Metab. Dispos.
29
200-205
2001
2
-
1
-
-
-
1
2
1
-
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1
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1
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1
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2
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1
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1
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2
1
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718453
Miyazawa
Roles of cytochrome P450 3A en ...
Homo sapiens, Rattus norvegicus
Xenobiotica
31
713-723
2001
2
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2
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2
3
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