BRENDA - Enzyme Database show
show all sequences of 1.14.14.59

Characterization of biosynthetic pathways for the production of the volatile homoterpenes DMNT and TMTT in Zea mays

Richter, A.; Schaff, C.; Zhang, Z.; Lipka, A.; Tian, F.; Koellner, T.; Schnee, C.; Preiss, S.; Irmisch, S.; Jander, G.; Boland, W.; Gershenzon, J.; Buckler, E.; Degenhardt, J.; Plant Cell 28, 2651-2665 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0142
-
(3S,6E)-nerolidol
pH 6.8, 30°C
Zea mays
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
B6ST66
enzyme catalyzes reactions of EC 1.14.14.58 and of EC 1.14.14.59
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2
-
746032
Zea mays
(3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
-
-
?
additional information
no substrate: linalool
746032
Zea mays
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0142
-
(3S,6E)-nerolidol
pH 6.8, 30°C
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2
-
746032
Zea mays
(3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
-
-
?
additional information
no substrate: linalool
746032
Zea mays
?
-
-
-
-
Expression
Organism
Commentary
Expression
Zea mays
low transcript levels in undamaged leaves, expression is strongly induced in response to simulated herbivory
additional information
General Information
General Information
Commentary
Organism
physiological function
gene disruption of CYP92C5 results in loss of (3E)-4,8-dimethylnona-1,3,7-triene production and reduced levels of (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene after simulated herbivory. In planta (3E)-4,8-dimethylnona-1,3,7-triene is primarily produced by isoform CYP92C5, whereas the majority of (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene is produced by cytochrome P450 monooxygenase, CYP92C6
Zea mays
General Information (protein specific)
General Information
Commentary
Organism
physiological function
gene disruption of CYP92C5 results in loss of (3E)-4,8-dimethylnona-1,3,7-triene production and reduced levels of (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene after simulated herbivory. In planta (3E)-4,8-dimethylnona-1,3,7-triene is primarily produced by isoform CYP92C5, whereas the majority of (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene is produced by cytochrome P450 monooxygenase, CYP92C6
Zea mays
Expression (protein specific)
Organism
Commentary
Expression
Zea mays
low transcript levels in undamaged leaves, expression is strongly induced in response to simulated herbivory
additional information
Other publictions for EC 1.14.14.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746032
Richter
Characterization of biosynthe ...
Zea mays
Plant Cell
28
2651-2665
2016
-
-
1
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
746284
Lee
Herbivore-induced and floral ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
107
21205-21210
2010
-
-
1
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1