BRENDA - Enzyme Database show
show all sequences of 1.14.14.59

Herbivore-induced and floral homoterpene volatiles are biosynthesized by a single P450 enzyme (CYP82G1) in Arabidopsis

Lee, S.; Badieyan, S.; Bevan, D.R.; Herde, M.; Gatz, C.; Tholl, D.; Proc. Natl. Acad. Sci. USA 107, 21205-21210 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Crystallization (Commentary)
Crystallization
Organism
modeling and substrate docking support an oxidative bond cleavage of the alcohol substrate via syn-elimination of the polar head, together with an allylic C-5 hydrogen atom
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0018
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LSF8
enzyme catalyzes reactions of EC 1.14.14.58 and of EC 1.14.14.59
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves
Arabidopsis thaliana
-
stem
constitutively expressed
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2
-
746284
Arabidopsis thaliana
(3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
Crystallization
Organism
modeling and substrate docking support an oxidative bond cleavage of the alcohol substrate via syn-elimination of the polar head, together with an allylic C-5 hydrogen atom
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0018
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves
Arabidopsis thaliana
-
stem
constitutively expressed
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2
-
746284
Arabidopsis thaliana
(3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
110
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
110
-
(3S,6E)-nerolidol
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Other publictions for EC 1.14.14.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746032
Richter
Characterization of biosynthe ...
Zea mays
Plant Cell
28
2651-2665
2016
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746284
Lee
Herbivore-induced and floral ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
107
21205-21210
2010
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