BRENDA - Enzyme Database show
show all sequences of 1.14.14.60

Cytochrome P450 promiscuity leads to a bifurcating biosynthetic pathway for tanshinones

Guo, J.; Ma, X.; Cai, Y.; Ma, Y.; Zhan, Z.; Zhou, Y.J.; Liu, W.; Guan, M.; Yang, J.; Cui, G.; Kang, L.; Yang, L.; Shen, Y.; Tang, J.; Lin, H.; Ma, X.; Jin, B.; Liu, Z.; Peters, R.J.; Zhao, Z.K.; Huang, L.; New Phytol. 210, 525-534 (2016)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salvia miltiorrhiza
A0A0Y0GRS3
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
expression is more abundant in the root than in aerial tissues
Salvia miltiorrhiza
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferruginol + [reduced NADPH-hemoprotein reductase] + O2
-
745904
Salvia miltiorrhiza
11-hydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
enzyme additionally oxidizes at C-7 to yield sugiol, reaction of EC 1.14.14.65
745904
Salvia miltiorrhiza
?
-
-
-
-
sugiol + [reduced NADPH-hemoprotein reductase] + O2
-
745904
Salvia miltiorrhiza
11-hydroxysugiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Salvia miltiorrhiza
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Salvia miltiorrhiza
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
expression is more abundant in the root than in aerial tissues
Salvia miltiorrhiza
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferruginol + [reduced NADPH-hemoprotein reductase] + O2
-
745904
Salvia miltiorrhiza
11-hydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
enzyme additionally oxidizes at C-7 to yield sugiol, reaction of EC 1.14.14.65
745904
Salvia miltiorrhiza
?
-
-
-
-
sugiol + [reduced NADPH-hemoprotein reductase] + O2
-
745904
Salvia miltiorrhiza
11-hydroxysugiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
cytochrome CYP76AH3 oxidizes ferruginol at two different carbon centers, and CYP76AK1 hydroxylates at C-20 of two of the resulting intermediates. Together, they convert ferruginol into 11,20-dihydroxy ferruginol and 11,20-dihydroxy sugiol en route to tanshinones. Silencing of CYP76AH3 leads to significant reduction in concentration of 11-hydroxyferruginol and 11-hydroxysugiol
Salvia miltiorrhiza
General Information (protein specific)
General Information
Commentary
Organism
physiological function
cytochrome CYP76AH3 oxidizes ferruginol at two different carbon centers, and CYP76AK1 hydroxylates at C-20 of two of the resulting intermediates. Together, they convert ferruginol into 11,20-dihydroxy ferruginol and 11,20-dihydroxy sugiol en route to tanshinones. Silencing of CYP76AH3 leads to significant reduction in concentration of 11-hydroxyferruginol and 11-hydroxysugiol
Salvia miltiorrhiza
Other publictions for EC 1.14.14.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746484
Ignea
Overcoming the plasticity of ...
Salvia pomifera
Sci. Rep.
7
8855
2017
-
1
1
1
2
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1
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1
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1
1
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1
2
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1
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-
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745860
Scheler
Elucidation of the biosynthes ...
Salvia fruticosa, Salvia rosmarinus
Nat. Commun.
7
12942
2016
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2
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2
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2
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745904
Guo
Cytochrome P450 promiscuity l ...
Salvia miltiorrhiza
New Phytol.
210
525-534
2016
-
-
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-
-
-
-
-
-
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-
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3
-
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1
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3
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1
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1
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1
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3
-
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1
1
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746312
Ignea
Carnosic acid biosynthesis el ...
Salvia pomifera
Proc. Natl. Acad. Sci. USA
113
3681-3686
2016
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1
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1
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1
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