BRENDA - Enzyme Database show
show all sequences of 1.14.14.61

Carnosic acid biosynthesis elucidated by a synthetic biology platform

Ignea, C.; Athanasakoglou, A.; Ioannou, E.; Georgantea, P.; Trikka, F.A.; Loupassaki, S.; Roussis, V.; Makris, A.M.; Kampranis, S.C.; Proc. Natl. Acad. Sci. USA 113, 3681-3686 (2016)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salvia pomifera
A0A0S1TPC7
-
-
Salvia rosmarinus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
glandular trichome
Salvia pomifera
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia rosmarinus
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia pomifera
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia rosmarinus
pisiferic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia pomifera
pisiferic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
CYP76AK6 is a multifunctional enzyme that catalyzes successive oxidation events at C-20 of the labdane skeleton
746312
Salvia rosmarinus
?
-
-
-
-
additional information
CYP76AK6 is a multifunctional enzyme that catalyzes successive oxidation events at C-20 of the labdane skeleton
746312
Salvia pomifera
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Salvia pomifera
cytochrome P450
-
Salvia rosmarinus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Salvia pomifera
cytochrome P450
-
Salvia rosmarinus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
glandular trichome
Salvia pomifera
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia rosmarinus
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia pomifera
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia rosmarinus
pisiferic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746312
Salvia pomifera
pisiferic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
CYP76AK6 is a multifunctional enzyme that catalyzes successive oxidation events at C-20 of the labdane skeleton
746312
Salvia rosmarinus
?
-
-
-
-
additional information
CYP76AK6 is a multifunctional enzyme that catalyzes successive oxidation events at C-20 of the labdane skeleton
746312
Salvia pomifera
?
-
-
-
-
Other publictions for EC 1.14.14.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745860
Scheler
Elucidation of the biosynthes ...
Salvia fruticosa, Salvia pomifera, Salvia rosmarinus
Nat. Commun.
7
12942
2016
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2
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3
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1
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6
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2
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1
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6
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746312
Ignea
Carnosic acid biosynthesis el ...
Salvia pomifera, Salvia rosmarinus
Proc. Natl. Acad. Sci. USA
113
3681-3686
2016
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4
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1
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6
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2
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2
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1
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6
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