BRENDA - Enzyme Database show
show all sequences of 1.14.14.63

Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus

Tamura, K.; Teranishi, Y.; Ueda, S.; Suzuki, H.; Kawano, N.; Yoshimatsu, K.; Saito, K.; Kawahara, N.; Muranaka, T.; Seki, H.; Plant Cell Physiol. 58, 874-884 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Platycodon grandiflorus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Platycodon grandiflorus
A0A1I9Q5Z0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Platycodon grandiflorus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
enzyme catalyzes the hydroxylation of beta-amyrin at C-16beta, plus further oxidation at C-28 to cochalic acid as a minor reaction
746048
Platycodon grandiflorus
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Platycodon grandiflorus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Platycodon grandiflorus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Platycodon grandiflorus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Platycodon grandiflorus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
enzyme catalyzes the hydroxylation of beta-amyrin at C-16beta, plus further oxidation at C-28 to cochalic acid as a minor reaction
746048
Platycodon grandiflorus
?
-
-
-
-
Other publictions for EC 1.14.14.63
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
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