BRENDA - Enzyme Database show
show all sequences of 1.14.14.69

Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.; Appl. Microbiol. Biotechnol. 99, 7549-7558 (2015)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa Japonica Group
Q6YV88
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
enzyme catalyzes the production of 3alpha-hydroxy-ent-cassadien-2-one via 2alpha-hydroxy-ent-cassadiene and ent-cassadien-2-one
744147
Oryza sativa Japonica Group
?
-
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2
-
744147
Oryza sativa Japonica Group
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
enzyme catalyzes the production of 3alpha-hydroxy-ent-cassadien-2-one via 2alpha-hydroxy-ent-cassadiene and ent-cassadien-2-one
744147
Oryza sativa Japonica Group
?
-
-
-
-
Other publictions for EC 1.14.14.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744525
Ye
In planta functions of cytoch ...
Oryza sativa Japonica Group
Biosci. Biotechnol. Biochem.
69
1021-1030
2018
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1
1
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744147
Kitaoka
Optimization of recombinant e ...
Oryza sativa Japonica Group
Appl. Microbiol. Biotechnol.
99
7549-7558
2015
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4
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744895
Wu
Parsing a multifunctional bio ...
Oryza sativa Japonica Group
FEBS Lett.
585
3446-3451
2011
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3
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