BRENDA - Enzyme Database show
show all sequences of 1.14.14.70

CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism

Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.; Plant Physiol. 158, 1418-1425 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain C41
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.0023
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.007
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
-
728514
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.013
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.017
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.022
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain C41
Oryza sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.0023
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.007
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
-
728514
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.013
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.017
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.022
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
6
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
9
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
6
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
9
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
Other publictions for EC 1.14.14.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729163
Wu
Picking sides: Distinct roles ...
Oryza sativa
Biochem. J.
454
209-216
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728514
Wang
CYP701A8: a rice ent-kaurene o ...
Oryza sativa
Plant Physiol.
158
1418-1425
2012
-
-
1
-
-
-
-
3
-
-
-
3
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3