BRENDA - Enzyme Database
show all sequences of 1.14.14.72

Identification of a drimenol synthase and drimenol oxidase from Persicaria hydropiper, involved in the biosynthesis of insect deterrent drimanes

Henquet, M.G.L.; Prota, N.; van der Hooft, J.J.J.; Varbanova-Herde, M.; Hulzink, R.J.M.; de Vos, M.; Prins, M.; de Both, M.T.J.; Franssen, M.C.R.; Bouwmeester, H.; Jongsma, M.; Plant J. 90, 1052-1063 (2017)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
drimenol
pH 7.5, 25C
Persicaria hydropiper
Organism
Organism
UniProt
Commentary
Textmining
Persicaria hydropiper
W0FKI0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
drimenol + [reduced NADPH-hemoprotein reductase] + O2
-
743475
Persicaria hydropiper
drimendiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
DOX1 catalyses the conversion from drimenol to drimendiol, and not the further oxidation into an aldehyde
743475
Persicaria hydropiper
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 56149, calculated
Persicaria hydropiper
Synonyms
Synonyms
Commentary
Organism
CYP76AJ1
-
Persicaria hydropiper
DOX1
-
Persicaria hydropiper
drimenol oxidase 1
-
Persicaria hydropiper
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Persicaria hydropiper
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Persicaria hydropiper
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
drimenol
pH 7.5, 25C
Persicaria hydropiper
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
drimenol + [reduced NADPH-hemoprotein reductase] + O2
-
743475
Persicaria hydropiper
drimendiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
DOX1 catalyses the conversion from drimenol to drimendiol, and not the further oxidation into an aldehyde
743475
Persicaria hydropiper
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56149, calculated
Persicaria hydropiper
General Information
General Information
Commentary
Organism
physiological function
coexpression of drimenol synthase with drimenol oxidase DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. Upon coexpression of drimenol synthase with drimenol oxidase DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives are observed
Persicaria hydropiper
General Information (protein specific)
General Information
Commentary
Organism
physiological function
coexpression of drimenol synthase with drimenol oxidase DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. Upon coexpression of drimenol synthase with drimenol oxidase DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives are observed
Persicaria hydropiper
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743475
Henquet
Identification of a drimenol ...
Persicaria hydropiper
Plant J.
90
1052-1063
2017
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