BRENDA - Enzyme Database show
show all sequences of 1.14.14.77

A novel cytochrome P450, CYP3201B1, is involved in (R)-mandelonitrile biosynthesis in a cyanogenic millipede

Yamaguchi, T.; Kuwahara, Y.; Asano, Y.; FEBS open bio 7, 335-347 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
coexpressed with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae
Chamberlinius hualienensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0066
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0069
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0092
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0158
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Chamberlinius hualienensis
no substrate: (E/Z)-phenylacetaldoxime
?
-
-
-
additional information
Chamberlinius hualienensis
no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene
?
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chamberlinius hualienensis
A0A1J1DVP9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrate: (E/Z)-phenylacetaldoxime
744919
Chamberlinius hualienensis
?
-
-
-
-
additional information
no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene
744919
Chamberlinius hualienensis
?
-
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Chamberlinius hualienensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
stable for 20 min
Chamberlinius hualienensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.71
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
1.09
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
1.12
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
3.07
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Chamberlinius hualienensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
7
-
Chamberlinius hualienensis
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Chamberlinius hualienensis
Cloned(Commentary) (protein specific)
Commentary
Organism
coexpressed with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae
Chamberlinius hualienensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Chamberlinius hualienensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0066
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0069
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0092
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
0.0158
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Chamberlinius hualienensis
no substrate: (E/Z)-phenylacetaldoxime
?
-
-
-
additional information
Chamberlinius hualienensis
no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene
?
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
Chamberlinius hualienensis
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrate: (E/Z)-phenylacetaldoxime
744919
Chamberlinius hualienensis
?
-
-
-
-
additional information
no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene
744919
Chamberlinius hualienensis
?
-
-
-
-
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
744919
Chamberlinius hualienensis
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Chamberlinius hualienensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
stable for 20 min
Chamberlinius hualienensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.71
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
1.09
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
1.12
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
3.07
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Chamberlinius hualienensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
7
-
Chamberlinius hualienensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
45
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
158
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
170
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
335
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
45
-
4-Hydroxyphenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
158
-
3-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
170
-
2-methyl phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
335
-
phenylacetonitrile
pH 6.0, 20°C
Chamberlinius hualienensis
Other publictions for EC 1.14.14.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744919
Yamaguchi
A novel cytochrome P450, CYP3 ...
Chamberlinius hualienensis
FEBS open bio
7
335-347
2017
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6
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7
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1
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1
4
1
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1
1
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1
1
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4
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1
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