BRENDA - Enzyme Database show
show all sequences of 1.14.14.80

Purification and cDNA cloning of human liver CYP4A fatty acid omega-hydroxylase

Kawashima, H.; Kusunose, E.; Kikuta, Y.; Kinoshita, H.; Tanaka, S.; Yamamoto, S.; Kishimoto, T.; Kusunose, M.; J. Biochem. 116, 74-80 (1994)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
59347
-
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q02928
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.7
-
substrate undecanoic acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
1
-
substrate lauric acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
3
-
substrate tridecanoic acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
8.5
-
substrate tridecanoic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
12
-
substrate undecanoic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
13.9
-
substrate isomyristic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
14.8
-
substrate lauric acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
24.5
-
substrate isolauric acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
isolauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
11-hydroxyisolauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
isomyristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
13-hydroxyisomyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
isoform CYP4A11 catalyzes the omega- and omega-1-hydroxylation of various fatty acids
734105
Homo sapiens
?
-
-
-
-
tridecanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
13-hydroxytridecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
undecanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
11-hydroxyundecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
59347
-
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.7
-
substrate undecanoic acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
1
-
substrate lauric acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
3
-
substrate tridecanoic acid, omega-1-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
8.5
-
substrate tridecanoic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
12
-
substrate undecanoic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
13.9
-
substrate isomyristic acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
14.8
-
substrate lauric acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
24.5
-
substrate isolauric acid, omega-hydroxylation, in nmol/min/nmol, pH 7.5, 37C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
isolauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
11-hydroxyisolauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
isomyristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
13-hydroxyisomyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
isoform CYP4A11 catalyzes the omega- and omega-1-hydroxylation of various fatty acids
734105
Homo sapiens
?
-
-
-
-
tridecanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
13-hydroxytridecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
undecanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
734105
Homo sapiens
11-hydroxyundecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 52000, SDS-PAGE, x * 59347, calculated
Homo sapiens
Other publictions for EC 1.14.14.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746320
Zhang
Ablation of cytochrome P450 o ...
Mus musculus
Proc. Natl. Acad. Sci. USA
114
3181-3185
2017
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746560
Uehara
Marmoset cytochrome P450 4A11 ...
Callithrix jacchus
Xenobiotica
47
553-561
2017
-
-
1
-
-
-
-
-
1
1
-
3
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
3
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744773
Han
Biochemical analysis of recom ...
Homo sapiens
Drug Metab. Pharmacokinet.
31
445-450
2016
1
-
1
-
3
-
-
17
-
1
-
3
-
1
-
-
1
-
-
2
-
-
3
1
1
-
-
17
1
-
-
3
-
-
-
1
-
1
3
-
3
-
-
-
-
17
-
1
-
3
-
-
-
1
-
2
-
-
3
1
1
-
-
17
1
-
-
-
-
-
-
-
18
18
746057
Bjelica
Fatty acid omega-hydroxylases ...
Solanum tuberosum
Plant Cell Rep.
35
2435-2448
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744772
Saito
Functional characterization o ...
Homo sapiens
Drug Metab. Pharmacokinet.
30
119-122
2015
-
-
1
-
9
-
-
7
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733174
Huang
Expression and characterizatio ...
Starmerella bombicola
Appl. Environ. Microbiol.
80
766-776
2014
-
-
-
-
-
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
744097
Huang
Expression and characterizati ...
Starmerella bombicola, Starmerella bombicola ATCC 22214
Appl. Environ. Microbiol.
80
766-776
2014
-
-
1
-
-
-
-
3
1
-
-
28
-
9
-
-
1
-
-
-
-
-
36
-
2
-
-
-
1
-
-
2
-
-
-
-
-
3
6
-
-
-
-
-
-
3
3
-
-
28
-
-
-
3
-
-
-
-
36
-
3
-
-
-
3
-
-
-
-
2
6
-
-
-
744323
Kim
Kinetic analysis of lauric ac ...
Homo sapiens
Biochemistry
53
6161-6172
2014
1
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703412
Nakano
Expression and characterizatio ...
Homo sapiens
Drug Metab. Dispos.
37
2119-2122
2009
-
-
1
-
-
-
1
3
1
-
1
-
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
3
1
-
1
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706321
Dobritsa
CYP704B1 is a long-chain fatty ...
Arabidopsis thaliana
Plant Physiol.
151
574-589
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
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7
-
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1
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-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
733891
Van Bogaert
Importance of the cytochrome P ...
Starmerella bombicola
FEMS Yeast Res.
9
87-94
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
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-
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-
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-
-
-
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-
-
-
1
1
1
1
-
-
734431
Gatica
P450 CYP2C epoxygenase and CYP ...
Rattus norvegicus
J. Lipid Res.
48
924-934
2007
-
1
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-
-
-
-
-
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
676666
Benveniste
Evolutionary relationship and ...
Arabidopsis thaliana
Plant Sci.
170
326-338
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
4
-
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1
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1
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4
-
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734950
Duan
Differential expression and ev ...
Arabidopsis thaliana
Plant Physiol.
137
1067-1081
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
7
-
-
5
-
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-
-
-
-
-
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15
-
-
5
-
-
-
-
-
-
-
-
-
2
-
-
8
-
-
285382
Hoch
Structural determination of th ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
373
63-71
2000
-
-
2
-
1
-
-
13
-
-
-
-
-
8
-
-
-
-
-
2
-
-
25
-
-
-
-
21
-
-
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
14
-
-
-
-
-
-
-
-
-
5
-
-
25
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
733243
Benveniste
CYP86A1 from Arabidopsis thali ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
243
688-693
1998
-
-
1
-
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-
1
-
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1
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6
-
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-
1
-
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-
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1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
734106
Hiratsuka
Sex and strain differences in ...
Mus musculus
J. Biochem.
119
340-345
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
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-
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-
-
1
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-
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-
-
-
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-
-
-
-
-
-
1
1
-
-
-
734105
Kawashima
Purification and cDNA cloning ...
Homo sapiens
J. Biochem.
116
74-80
1994
-
-
1
-
-
-
-
-
1
-
2
-
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1
-
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1
-
-
2
8
-
6
1
-
-
-
-
-
-
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-
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-
1
-
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1
-
2
-
-
-
-
1
-
2
8
-
6
1
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