Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes | Osteospermum hybrid cultivar |
Protein Variants | Comment | Organism |
---|---|---|
F484Y/S487T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
F48Y | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
additional information | generation of chimeric constructs consisting of cDNA fragments of Osteospermum hybrida F3'5'H and Gerbera hybrida F3'H, EC 1.14.13.21, overview | Osteospermum hybrid cultivar |
S487A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
S487Y | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a cytochrome P450 enzyme | Osteospermum hybrid cultivar |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
apigenin + [reduced NADPH-hemoprotein reductase] + O2 | Osteospermum hybrid cultivar | - |
? | - |
? | |
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Osteospermum hybrid cultivar | - |
dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | Osteospermum hybrid cultivar | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | ? | - |
? | |
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Osteospermum hybrid cultivar | - |
(2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Osteospermum hybrid cultivar | Q304Q4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of recombinant chimeric mutant enzymes, overview | Osteospermum hybrid cultivar |
0.0000026 | - |
recombinant wild-type F3'5'H | Osteospermum hybrid cultivar |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Osteospermum hybrid cultivar | ? | - |
? | |
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Osteospermum hybrid cultivar | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | preferred substrate of the wild-type enzyme | Osteospermum hybrid cultivar | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | Osteospermum hybrid cultivar | ? | - |
? | |
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Osteospermum hybrid cultivar | (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3'5'H | - |
Osteospermum hybrid cultivar |
flavonoid 3',5'-hydroxylase | - |
Osteospermum hybrid cultivar |
More | the enzyme belongs to the subfamilies CYP75A of the superfamily of cytochrome P450-dependent monooxygenases | Osteospermum hybrid cultivar |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Osteospermum hybrid cultivar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Osteospermum hybrid cultivar |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | a cytochrome P450 enzyme | Osteospermum hybrid cultivar |