BRENDA - Enzyme Database show
show all sequences of 1.14.14.81

The B-ring hydroxylation pattern of anthocyanins can be determined through activity of the flavonoid 3-hydroxylase on leucoanthocyanidins

Schwinn, K.; Miosic, S.; Davies, K.; Thill, J.; Gotame, T.P.; Stich, K.; Halbwirth, H.; Planta 240, 1003-1010 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
0.001
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
0.0058
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
0.009
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Billardiera heterophylla
U3LXJ8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
tricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
myricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
pentahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.6
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
13.4
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
17
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
81.4
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Billardiera heterophylla
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
0.001
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
0.0058
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
0.009
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
tricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
myricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
746151
Billardiera heterophylla
pentahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.6
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
13.4
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
17
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
81.4
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Billardiera heterophylla
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
900
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
1700
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
2300
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
10000
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
900
-
dihydrokaempferol
pH 7.0, 25°C
Billardiera heterophylla
1700
-
kaempferol
pH 7.0, 25°C
Billardiera heterophylla
2300
-
apigenin
pH 7.0, 25°C
Billardiera heterophylla
10000
-
naringenin
pH 7.0, 25°C
Billardiera heterophylla
Other publictions for EC 1.14.14.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744460
Wang
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Identification and functional ...
Phalaenopsis hybrid cultivar, Phalaenopsis sp.
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62
45-54
2018
-
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1
-
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-
3
-
2
-
-
-
-
-
1
-
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3
-
-
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-
1
-
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-
1
1
-
-
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3
-
-
-
-
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1
-
-
3
-
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3
3
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746005
Hammerbacher
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Gallocatechin biosynthesis vi ...
Picea abies
Phytochemistry
148
78-86
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-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
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-
3
-
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3
-
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3
-
-
-
-
-
-
-
-
-
1
1
1
1
-
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746159
Jin
-
Functional natural allelic va ...
Camellia sinensis, Camellia sinensis var. sinensis
Planta
245
523-538
2017
-
2
2
-
2
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
2
2
2
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
746151
Schwinn
The B-ring hydroxylation patt ...
Billardiera heterophylla
Planta
240
1003-1010
2014
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
4
1
-
-
-
-
-
-
-
4
4
745025
Huang
Isolation and molecular chara ...
Epimedium sagittatum
Gene
497
125-130
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
745636
Ishiguro
Functional analysis of Antirr ...
Antirrhinum kelloggii
J. Plant Res.
125
451-456
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
728200
Whang
-
Molecular analysis of anthocya ...
Dendrobium moniliforme
J. Plant Biol.
54
209-218
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711612
Takahashi
A new allele of flower color g ...
Glycine soja, Glycine soja B09121, Glycine soja Clark
BMC Plant Biol.
10
155
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711613
Olsen
Identification and characteris ...
Solanum lycopersicum
BMC Plant Biol.
10
21
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
695439
Kobayashi
Low expression of flavonoid 3, ...
Vitis vinifera
Am. J. Enol. Vitic.
60
362-367
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
699811
Nakatsuka
Genetic engineering of novel f ...
Gentiana triflora
J. Plant Physiol.
167
231-237
2009
-
-
1
-
1
-
1
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
700869
Akagi
Expression balances of structu ...
Diospyros kaki
Planta
230
899-915
2009
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
685832
Castellarin
Transcriptional control of ant ...
Vitis vinifera
BMC Plant Biol.
7
46
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
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-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686737
Seitz
Identification of the molecula ...
Osteospermum hybrid cultivar
FEBS Lett.
581
3429-3434
2007
-
-
1
-
5
-
-
-
-
1
-
4
-
5
-
-
-
-
-
-
2
-
5
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
2
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
688630
Seo
-
Co-expression of flavonoid 3', ...
no activity in Chrysanthemum x morifolium, Petunia sp.
J. Plant Biol.
50
626-631
2007
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689425
Seitz
Redirection of anthocyanin syn ...
Osteospermum hybrid cultivar
Phytochemistry
68
824-833
2007
-
-
-
-
1
-
-
-
-
-
-
3
-
6
-
-
-
-
-
3
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
689479
Castellarin
Transcriptional regulation of ...
Vitis vinifera
Plant Cell Environ.
30
1381-1399
2007
1
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689488
Katsumoto
Engineering of the rose flavon ...
no activity in Rosa hybrida, Viola sp.
Plant Cell Physiol.
48
1589-1600
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
1
-
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-
1
-
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-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689681
Castellarin
Water deficits accelerate ripe ...
Vitis vinifera
Planta
227
101-112
2007
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
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1
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1
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1
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2
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-
-
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-
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-
672803
Castellarin
Colour variation in red grapev ...
Vitis vinifera
BMC Genomics
7
12
2006
1
-
1
-
-
-
-
-
-
-
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15
-
5
-
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4
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15
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5
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5
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15
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20
-
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15
-
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-
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-
675792
Leonard
Functional expression of a P45 ...
Catharanthus roseus
Metab. Eng.
8
172-181
2006
-
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1
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1
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1
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676011
Nakatsuka
Two different transposable ele ...
Gentiana scabra, Gentiana triflora
Mol. Genet. Genomics
275
231-241
2006
-
-
2
-
2
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2
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5
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1
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2
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2
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2
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2
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1
-
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2
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-
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676545
Seitz
Cloning, functional identifica ...
Callistephus chinensis, Delphinium grandiflorum, Glandularia x hybrida, Glycine max, Gossypium hirsutum, Lycianthes rantonnei, Nierembergia sp., Osteospermum hybrid cultivar, Pericallis cruenta, Petunia x hybrida, Solanum melongena, Solanum tuberosum, Torenia hybrid cultivar, Vinca major
Plant Mol. Biol.
61
365-381
2006
-
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14
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14
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14
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18
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28
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85
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14
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14
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14
-
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14
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14
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28
-
-
85
-
14
-
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14
-
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676595
Bogs
Identification of the flavonoi ...
Vitis vinifera
Plant Physiol.
140
279-291
2006
-
1
1
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1
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-
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1
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3
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3
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2
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3
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1
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1
1
1
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1
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1
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3
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2
-
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3
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676670
Jeong
Expression of the flavonoid 3' ...
Vitis vinifera
Plant Sci.
170
61-69
2006
-
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1
-
-
-
-
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-
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3
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1
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6
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3
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1
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3
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6
-
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3
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660110
Robbins
A comparison of two strategies ...
Eustoma exaltatum subsp. russellianum
Phytochemistry
66
991-999
2005
-
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1
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-
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6
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1
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660543
Jung
The potato P locus codes for f ...
Solanum tuberosum
Theor. Appl. Genet.
110
269-275
2005
-
-
-
-
-
-
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1
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7
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1
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1
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1
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1
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1
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656986
Mori
Heterologous expression of the ...
Vinca major
Plant Cell Rep.
22
415-421
2004
-
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1
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5
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3
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1
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3
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656985
Shimada
-
Genetic engineering of the ant ...
Petunia x hybrida
Plant Cell Rep.
20
456-462
2001
-
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1
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1
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1
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1
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1
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1
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1
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-
-
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-
655537
Shimada
Expression of chimeric P450 ge ...
Eustoma exaltatum subsp. russellianum, Petunia x hybrida
FEBS Lett.
461
241-245
1999
-
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2
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2
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6
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1
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2
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2
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2
2
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2
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1
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2
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-
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-
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657175
de Vetten
A cytochrome b5 is required fo ...
Petunia sp.
Proc. Natl. Acad. Sci. USA
96
778-783
1999
-
-
-
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1
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1
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2
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1
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1
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1
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1
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657026
Menting
Characterization of flavonoid ...
Petunia x hybrida
Plant Physiol.
106
633-642
1994
1
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1
33
-
1
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1
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1
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2
4
-
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1
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1
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1
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1
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1
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33
-
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1
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1
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2
4
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1
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