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Literature summary for 1.14.14.82 extracted from
- Microsomal flavonoid 3'-monooxygenase from maize seedlings (1986), Plant Physiol., 80, 483-486.
General Stability
| General Stability | Organism |
|---|---|
| sucrose or glycerol plus dithiothreitol stabilize during purification and storage at -70°C | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha,alpha'-dipyridyl | partial | Zea mays |  |
| CO | inhibition reduced by irradiation with 450 nm light during incubation | Zea mays | |
| cytochrome c | - |
Zea mays | |
| FeCl3 | - |
Zea mays | |
| N-ethylmaleimide | - |
Zea mays | |
| NADP+ | - |
Zea mays | |
| NaN3 | - |
Zea mays | |
| p-chloromercuribenzoate | partial | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Zea mays | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, sucrose or glycerol plus dithiothreitol stabilize during purification and storage | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | luteolin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | no activity | Zea mays | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| kaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | quercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
10 min, activity destroyed | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
sharp drop in activity on either side of the optimum of pH 8.5 | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | 0.21 nmol per mg of protein | Zea mays |
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Release 2023.1 (January 2023)
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