BRENDA - Enzyme Database show
show all sequences of 1.14.14.83

Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

Collu, G.; Unver, N.; Peltenburg-Looman, A.M.G.; van der Heijden, R.; Verpoorte, R.; Memelink, J.; FEBS Lett. 508, 215-220 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Catharanthus roseus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56300
-
x * 56300, SDS-PAGE
Catharanthus roseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
gene CYP76B6
-
Purification (Commentary)
Commentary
Organism
native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity
Catharanthus roseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
715004
Catharanthus roseus
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 56300, SDS-PAGE
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Catharanthus roseus
isoelectric focusing
-
8.7
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Catharanthus roseus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56300
-
x * 56300, SDS-PAGE
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity
Catharanthus roseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
715004
Catharanthus roseus
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56300, SDS-PAGE
Catharanthus roseus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Catharanthus roseus
isoelectric focusing
-
8.7
Expression
Organism
Commentary
Expression
Catharanthus roseus
the stress hormone methyljasmonate strongly induces G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus cell culture
up
General Information
General Information
Commentary
Organism
physiological function
geraniol 10-hydroxylase is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. G10H plays a key regulatory role in terpenoid indole alkaloid biosynthesis
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
geraniol 10-hydroxylase is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. G10H plays a key regulatory role in terpenoid indole alkaloid biosynthesis
Catharanthus roseus
Expression (protein specific)
Organism
Commentary
Expression
Catharanthus roseus
the stress hormone methyljasmonate strongly induces G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus cell culture
up
Other publictions for EC 1.14.14.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746070
Balusamy
-
Overexpression of geraniol 10 ...
Panax ginseng
Plant Growth Regul.
81
305-316
2017
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1
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1
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1
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5
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1
1
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2
2
2
2
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743812
Pandey
Fungal endophytes of Catharan ...
Catharanthus roseus
Sci. Rep.
6
26583
2016
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1
1
1
1
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743431
Sintupachee
Functional expression of a pu ...
Croton stellatopilosus, Croton stellatopilosus Ohba
Phytochemistry
118
204-215
2015
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-
1
-
-
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1
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3
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8
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1
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9
1
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5
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6
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3
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2
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9
2
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3
-
3
6
-
-
-
746441
Cui
Co-overexpression of geraniol ...
Catharanthus roseus
Sci. Rep.
5
8227
2015
-
3
1
-
1
-
-
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1
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3
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1
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1
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1
1
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1
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1
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1
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2
2
-
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743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
-
1
-
-
-
-
-
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1
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2
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2
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1
1
1
1
-
-
745686
Hoefer
Geraniol hydroxylase and hydr ...
Catharanthus roseus
Metab. Eng.
20
221-232
2013
-
-
1
1
-
-
-
-
-
-
-
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4
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2
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1
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2
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1
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1
1
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718240
Huang
Differential expression and fu ...
Catharanthus roseus
Plant Sci.
190
16-23
2012
-
-
1
-
1
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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2
2
-
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746239
Pan
Overexpression of ORCA3 and G ...
Catharanthus roseus
PLoS ONE
7
e43038
2012
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1
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1
1
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715234
Sung
Functional expression of geran ...
Catharanthus roseus
J. Agric. Food Chem.
59
4637-4643
2011
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1
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1
1
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1
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1
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1
1
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1
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2
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1
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1
1
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1
1
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1
1
716475
Poutrain
Molecular cloning and characte ...
Catharanthus roseus
Plant Biol.
13
36-41
2011
-
-
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1
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2
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2
2
2
2
-
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714525
Wang
Cloning and functional analysi ...
Swertia mussotii
Biosci. Biotechnol. Biochem.
74
1583-1590
2010
-
-
1
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-
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1
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1
2
-
5
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3
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3
1
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1
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1
1
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2
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3
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3
1
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1
3
3
1
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716102
Peebles
The expression of 1-deoxy-d-xy ...
Catharanthus roseus
Metab. Eng.
13
234-240
2010
-
-
1
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1
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2
2
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716501
Wang
Overexpression of G10H and ORC ...
Catharanthus roseus
Plant Cell Rep.
29
887-894
2010
-
-
1
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1
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1
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1
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1
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1
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2
2
2
2
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716447
Oudin
-
The iridoid pathway in Cathara ...
Catharanthus roseus
Phytochem. Rev.
6
259-276
2007
-
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1
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2
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1
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2
2
2
2
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744382
Suttipanta
Promoter analysis of the Cath ...
Catharanthus roseus
Biochim. Biophys. Acta
1769
139-148
2007
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1
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1
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718213
Burlat
Co-expression of three MEP pat ...
Catharanthus roseus
Plant J.
38
131-141
2004
-
-
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6
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6
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1
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3
3
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718237
Collu
-
Activity of the cytochrome P45 ...
Catharanthus roseus
Plant Sci.
162
165-172
2002
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1
2
2
1
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715004
Collu
Geraniol 10-hydroxylase, a cyt ...
Catharanthus roseus
FEBS Lett.
508
215-220
2001
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1
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1
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1
1
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4
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1
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1
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1
1
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1
1
1
1
1
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716446
Collu
-
Determination of the activity ...
Catharanthus roseus
Phytochem. Anal.
10
314-318
1999
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1
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3
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1
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1
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1
1
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717532
Hallahan
Cytochrome P-450 in plant/inse ...
Catharanthus roseus, Nepeta racemosa
Drug Metabol. Drug Interact.
12
369-382
1995
-
-
-
-
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3
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6
-
2
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3
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12
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2
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2
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3
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6
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3
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12
-
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6
6
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715730
Meijer
-
Purification of the cytochrome ...
Catharanthus roseus, no activity in Tabernaemontana pandacaqui, Tabernaemontana divaricata
J. Chromatogr. A
635
237-249
1993
-
-
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2
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1
4
-
3
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1
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1
2
1
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4
1
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2
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4
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4
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2
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1
4
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1
1
2
1
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4
1
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2
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2
2
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744287
Madyastha
Characterization of a cytochr ...
Catharanthus roseus
Biochemistry
15
1097-1102
1976
1
-
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5
2
1
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2
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3
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1
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1
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2
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-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
2
1
-
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-