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Literature summary for 1.14.14.83 extracted from
- Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis (2001), FEBS Lett., 508, 215-220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation | Catharanthus roseus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Catharanthus roseus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56300 | - |
x * 56300, SDS-PAGE | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
gene CYP76B6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56300, SDS-PAGE | Catharanthus roseus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP76B6 | - |
Catharanthus roseus |
| G10H | - |
Catharanthus roseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Catharanthus roseus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Catharanthus roseus | isoelectric focusing | - |
8.7 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Catharanthus roseus | the stress hormone methyljasmonate strongly induces G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus cell culture | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. G10H plays a key regulatory role in terpenoid indole alkaloid biosynthesis | Catharanthus roseus |
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Release 2023.1 (January 2023)
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