Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Catharanthus roseus | 16020 | - |
membrane | - |
Tabernaemontana divaricata | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Tabernaemontana divaricata | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
nerol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | cis-isomer of geraniol | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
nerol + [reduced NADPH-hemoprotein reductase] + O2 | Tabernaemontana divaricata | cis-isomer of geraniol | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
no activity in Tabernaemontana pandacaqui | - |
- |
- |
Tabernaemontana divaricata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4 | Catharanthus roseus |
Renatured (Comment) | Organism |
---|---|
reconstitution of the purified enzyme by addition of NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and lipid extract | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
cell suspension culture | during the first week of growth, no activity in the stationary growth phase | Tabernaemontana divaricata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.66 | - |
purified enzyme, pH 7.6, temperature not specified in the publication | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Tabernaemontana divaricata | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
nerol + [reduced NADPH-hemoprotein reductase] + O2 | cis-isomer of geraniol | Catharanthus roseus | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
nerol + [reduced NADPH-hemoprotein reductase] + O2 | cis-isomer of geraniol | Tabernaemontana divaricata | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Catharanthus roseus |
Synonyms | Comment | Organism |
---|---|---|
G10H | - |
Catharanthus roseus |
G10H | - |
Tabernaemontana divaricata |
monoterpene hydroxylase | - |
Catharanthus roseus |
monoterpene hydroxylase | - |
Tabernaemontana divaricata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Catharanthus roseus |
7.6 | - |
assay at | Tabernaemontana divaricata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Tabernaemontana divaricata | |
cytochrome P450 | 4.72 nmol/mg | Catharanthus roseus | |
FAD | - |
Catharanthus roseus | |
FAD | - |
Tabernaemontana divaricata |
General Information | Comment | Organism |
---|---|---|
additional information | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase | Catharanthus roseus |
additional information | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase | Tabernaemontana divaricata |