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Literature summary for 1.14.14.83 extracted from

  • Meijer, A.H.; De Waal, A.; Verpoorte, R.
    Purification of the cytochrome P-450 enzyme geraniol 10-hydroxylase from cell cultures of Catharanthus roseus (1993), J. Chromatogr. A, 635, 237-249.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Catharanthus roseus 16020
-
membrane
-
Tabernaemontana divaricata 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, SDS-PAGE Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Tabernaemontana divaricata
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
nerol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus cis-isomer of geraniol 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
nerol + [reduced NADPH-hemoprotein reductase] + O2 Tabernaemontana divaricata cis-isomer of geraniol 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-
no activity in Tabernaemontana pandacaqui
-
-
-
Tabernaemontana divaricata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4 Catharanthus roseus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of the purified enzyme by addition of NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and lipid extract Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Catharanthus roseus
-
cell suspension culture during the first week of growth, no activity in the stationary growth phase Tabernaemontana divaricata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.66
-
purified enzyme, pH 7.6, temperature not specified in the publication Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
Tabernaemontana divaricata 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
nerol + [reduced NADPH-hemoprotein reductase] + O2 cis-isomer of geraniol Catharanthus roseus 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
nerol + [reduced NADPH-hemoprotein reductase] + O2 cis-isomer of geraniol Tabernaemontana divaricata 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Catharanthus roseus

Synonyms

Synonyms Comment Organism
G10H
-
Catharanthus roseus
G10H
-
Tabernaemontana divaricata
monoterpene hydroxylase
-
Catharanthus roseus
monoterpene hydroxylase
-
Tabernaemontana divaricata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Catharanthus roseus
7.6
-
assay at Tabernaemontana divaricata

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Tabernaemontana divaricata
cytochrome P450 4.72 nmol/mg Catharanthus roseus
FAD
-
Catharanthus roseus
FAD
-
Tabernaemontana divaricata

General Information

General Information Comment Organism
additional information geraniol 10-hydroxylase is a cytochrome P450 monooxygenase Catharanthus roseus
additional information geraniol 10-hydroxylase is a cytochrome P450 monooxygenase Tabernaemontana divaricata