Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda SF9 cells, co-expression with NADPH:cytochrome P450 reductase NfCPR2 from Nothapodytes foetida and CPR2 from Arabidopsis thaliana | Catharanthus roseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | microsomes isolated from insect cells co-expressing Nothapodytes foetida NfCPR2 and Catharanthus roseus geraniol 10-hydroxylase show enhanced the production of eriodictyol from naringenin approximately 11fold relative to control G10H-only insect cells, indicating the supportive role of NfCPR2 for G10H activity in insect cells. Addition of FAD and FMN up to 0.01 mM does not increase the supportive function of the NfCPR2 for G10H activity | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
(6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | Q8VWZ7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G10H | - |
Catharanthus roseus |
geraniol 10-hydroxylase | - |
Catharanthus roseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the cytochrome-P450 monooxygenase family | Catharanthus roseus |
metabolism | the enzyme is involved in the biosynthesis of secologanin from geraniol, overview | Catharanthus roseus |