BRENDA - Enzyme Database show
show all sequences of 1.14.14.83

Overexpression of geraniol 10-hydroxylase from Panax ginseng conferred enhanced resistance to Pseudomonas syringae in Arabidopsis

Balusamy, S.; Rahimi, S.; Cho, Y.; Senthil, K.; Yang, D.; Plant Growth Regul. 81, 305-316 (2017)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene G10H, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. Recombinant overexpression of the ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis thaliana ecotype CS60000, from vector pCAMBIA1390 under control of promoter CaMV 35S, causes terpenoid indole alkaloid dihydrositsirikine production and also confers enhanced resistance to Pseudomonas syringae pv. tomato DC3000
Panax ginseng
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
G10H is a transmembrane protein containing a transmembrane helix at the N-terminus
Panax ginseng
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
moderate enzyme expression level
Panax ginseng
-
leaf
low enzyme expression level
Panax ginseng
-
additional information
tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses
Panax ginseng
-
root
high enzyme expression level
Panax ginseng
-
stem
moderate enzyme expression level
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
746070
Panax ginseng
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
secondary structure and three-dimensional structure analysis, overview
Panax ginseng
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P450 monooxygenase
Panax ginseng
NADPH
-
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
gene G10H, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. Recombinant overexpression of the ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis thaliana ecotype CS60000, from vector pCAMBIA1390 under control of promoter CaMV 35S, causes terpenoid indole alkaloid dihydrositsirikine production and also confers enhanced resistance to Pseudomonas syringae pv. tomato DC3000
Panax ginseng
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P450 monooxygenase
Panax ginseng
NADPH
-
Panax ginseng
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
G10H is a transmembrane protein containing a transmembrane helix at the N-terminus
Panax ginseng
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
moderate enzyme expression level
Panax ginseng
-
leaf
low enzyme expression level
Panax ginseng
-
additional information
tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses
Panax ginseng
-
root
high enzyme expression level
Panax ginseng
-
stem
moderate enzyme expression level
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
746070
Panax ginseng
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
secondary structure and three-dimensional structure analysis, overview
Panax ginseng
Expression
Organism
Commentary
Expression
Panax ginseng
tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses
additional information
Panax ginseng
the expression of PgG10H is differentially induced in ginseng, not only during Pseudomonas syringae infection and wounding but also after exposure to methyl jasmonate and salt stress
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the secoiridoid pathway and the biosynthesis of terpenoid indole alkaloid via strictosidine
Panax ginseng
additional information
molecular docking is used for accurate prediction of protein-ligand interaction geometries at molecular level, molecular interaction studies, overview. Geraniol interacts with G10H residues Ala303 and Thr307 from the central helix I
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the secoiridoid pathway and the biosynthesis of terpenoid indole alkaloid via strictosidine
Panax ginseng
additional information
molecular docking is used for accurate prediction of protein-ligand interaction geometries at molecular level, molecular interaction studies, overview. Geraniol interacts with G10H residues Ala303 and Thr307 from the central helix I
Panax ginseng
Expression (protein specific)
Organism
Commentary
Expression
Panax ginseng
tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses
additional information
Panax ginseng
the expression of PgG10H is differentially induced in ginseng, not only during Pseudomonas syringae infection and wounding but also after exposure to methyl jasmonate and salt stress
up
Other publictions for EC 1.14.14.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746070
Balusamy
-
Overexpression of geraniol 10 ...
Panax ginseng
Plant Growth Regul.
81
305-316
2017
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2
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743812
Pandey
Fungal endophytes of Catharan ...
Catharanthus roseus
Sci. Rep.
6
26583
2016
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1
1
1
1
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743431
Sintupachee
Functional expression of a pu ...
Croton stellatopilosus, Croton stellatopilosus Ohba
Phytochemistry
118
204-215
2015
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8
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2
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3
6
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746441
Cui
Co-overexpression of geraniol ...
Catharanthus roseus
Sci. Rep.
5
8227
2015
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3
1
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2
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743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
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1
1
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1
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745686
Hoefer
Geraniol hydroxylase and hydr ...
Catharanthus roseus
Metab. Eng.
20
221-232
2013
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1
1
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4
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1
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1
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718240
Huang
Differential expression and fu ...
Catharanthus roseus
Plant Sci.
190
16-23
2012
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2
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746239
Pan
Overexpression of ORCA3 and G ...
Catharanthus roseus
PLoS ONE
7
e43038
2012
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1
1
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715234
Sung
Functional expression of geran ...
Catharanthus roseus
J. Agric. Food Chem.
59
4637-4643
2011
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1
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1
1
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1
1
716475
Poutrain
Molecular cloning and characte ...
Catharanthus roseus
Plant Biol.
13
36-41
2011
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2
2
2
2
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714525
Wang
Cloning and functional analysi ...
Swertia mussotii
Biosci. Biotechnol. Biochem.
74
1583-1590
2010
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1
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3
1
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716102
Peebles
The expression of 1-deoxy-d-xy ...
Catharanthus roseus
Metab. Eng.
13
234-240
2010
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2
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716501
Wang
Overexpression of G10H and ORC ...
Catharanthus roseus
Plant Cell Rep.
29
887-894
2010
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2
2
2
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716447
Oudin
-
The iridoid pathway in Cathara ...
Catharanthus roseus
Phytochem. Rev.
6
259-276
2007
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744382
Suttipanta
Promoter analysis of the Cath ...
Catharanthus roseus
Biochim. Biophys. Acta
1769
139-148
2007
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718213
Burlat
Co-expression of three MEP pat ...
Catharanthus roseus
Plant J.
38
131-141
2004
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3
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718237
Collu
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Activity of the cytochrome P45 ...
Catharanthus roseus
Plant Sci.
162
165-172
2002
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715004
Collu
Geraniol 10-hydroxylase, a cyt ...
Catharanthus roseus
FEBS Lett.
508
215-220
2001
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1
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716446
Collu
-
Determination of the activity ...
Catharanthus roseus
Phytochem. Anal.
10
314-318
1999
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1
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1
1
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717532
Hallahan
Cytochrome P-450 in plant/inse ...
Catharanthus roseus, Nepeta racemosa
Drug Metabol. Drug Interact.
12
369-382
1995
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3
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6
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12
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12
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6
6
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715730
Meijer
-
Purification of the cytochrome ...
Catharanthus roseus, no activity in Tabernaemontana pandacaqui, Tabernaemontana divaricata
J. Chromatogr. A
635
237-249
1993
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1
4
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3
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1
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1
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744287
Madyastha
Characterization of a cytochr ...
Catharanthus roseus
Biochemistry
15
1097-1102
1976
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