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Literature summary for 1.14.14.83 extracted from

  • Cui, L.; Ni, X.; Ji, Q.; Teng, X.; Yang, Y.; Wu, C.; Zekria, D.; Zhang, D.; Kai, G.
    Co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila (2015), Sci. Rep., 5, 8227 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus
pharmacology co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus
synthesis introduction of genes encoding strictosidine synthase (STR) and geraniol 8-hydroxylase (G10H), into Ophiorrhiza pumila hairy roots. Overexpression of individual G10H significantly improves camptothecin prdouction. Cooverexpression of G10H and STR genes causes a 56% increase on the yields of camptothecin. Camptothecin extracted from different lines shows similar anti-tumor activity Catharanthus roseus

Cloned(Commentary)

Cloned (Comment) Organism
co-overexpression of geraniol-10-hydroxylase and strictosidine synthase in Ophiorrhiza pumila Catharanthus roseus
gene CrG10H, recombinant expression in transgenic Ophiorrhiza pumila hairy roots using Agrobacterium rhizogenes strain C58C1 for transfection Catharanthus roseus

Protein Variants

Protein Variants Comment Organism
additional information overexpression of individual geraniol 10-hydroxylase, G10H (G lines), significantly improves camptothecin (CPT) production with respect to non-transgenic hairy root cultures (NC line) and single strictosidine synthase (STR, EC 4.3.3.2) overexpressing lines (S lines), indicating that G10H plays a more important role in stimulating CPT accumulation than STR in Ophiorrhiza pumila. Co-overexpression of G10H and STR genes (SG Lines) causes a 56% increase on the yields of CPT compared to NC line and single gene transgenic lines, show that simultaneous introduction of G10H and STR can produce a synergistic effect on CPT biosynthesis in Ophiorrhiza pumila. camptothecin accumulates in the hairy roots. G10H is a key regulation target gene for metabolic engineering of CPT biosynthetic pathway Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus Q8VWZ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP76B6 UniProt Catharanthus roseus
G10H
-
Catharanthus roseus
geraniol 10-hydroxylase
-
Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Catharanthus roseus

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first committed step in the camptothecin biosynthetic pathway and a key regulation point of the campothecin biosynthetic pathway Catharanthus roseus
physiological function the enzyme plays an important role in stimulating campothecin accumulation, capothecin has anti-tumor activity Catharanthus roseus