Application | Comment | Organism |
---|---|---|
drug development | co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila | Catharanthus roseus |
pharmacology | co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila | Catharanthus roseus |
synthesis | introduction of genes encoding strictosidine synthase (STR) and geraniol 8-hydroxylase (G10H), into Ophiorrhiza pumila hairy roots. Overexpression of individual G10H significantly improves camptothecin prdouction. Cooverexpression of G10H and STR genes causes a 56% increase on the yields of camptothecin. Camptothecin extracted from different lines shows similar anti-tumor activity | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
co-overexpression of geraniol-10-hydroxylase and strictosidine synthase in Ophiorrhiza pumila | Catharanthus roseus |
gene CrG10H, recombinant expression in transgenic Ophiorrhiza pumila hairy roots using Agrobacterium rhizogenes strain C58C1 for transfection | Catharanthus roseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of individual geraniol 10-hydroxylase, G10H (G lines), significantly improves camptothecin (CPT) production with respect to non-transgenic hairy root cultures (NC line) and single strictosidine synthase (STR, EC 4.3.3.2) overexpressing lines (S lines), indicating that G10H plays a more important role in stimulating CPT accumulation than STR in Ophiorrhiza pumila. Co-overexpression of G10H and STR genes (SG Lines) causes a 56% increase on the yields of CPT compared to NC line and single gene transgenic lines, show that simultaneous introduction of G10H and STR can produce a synergistic effect on CPT biosynthesis in Ophiorrhiza pumila. camptothecin accumulates in the hairy roots. G10H is a key regulation target gene for metabolic engineering of CPT biosynthetic pathway | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | Q8VWZ7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP76B6 | UniProt | Catharanthus roseus |
G10H | - |
Catharanthus roseus |
geraniol 10-hydroxylase | - |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first committed step in the camptothecin biosynthetic pathway and a key regulation point of the campothecin biosynthetic pathway | Catharanthus roseus |
physiological function | the enzyme plays an important role in stimulating campothecin accumulation, capothecin has anti-tumor activity | Catharanthus roseus |