BRENDA - Enzyme Database show
show all sequences of 1.14.14.84

Chemical probes into the active centre of a heme thiolate monoxygenase

Bhattacharyya, P.K.; Samanta, T.B.; Ullah, A.H.J.; Gunsalus, I.C.; Proc. Indian Acad. Sci. Chem. Sci. 93, 1289-1304 (1984)
No PubMed abstract available

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
PpG777
-
Pseudomonas putida PpG777
-
PpG777
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
both isomers of linalool, strict substrate specificity: geraniol, nerol or citronellol are not hydroxylated
390112
Pseudomonas putida
(E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390112
Pseudomonas putida
?
3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
both isomers of linalool, strict substrate specificity: geraniol, nerol or citronellol are not hydroxylated
390112
Pseudomonas putida PpG777
(E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390112
Pseudomonas putida PpG777
?
6-methyl-hex-5-en-2-ol + + [reduced NADPH-hemoprotein reductase] + O2
-
390112
Pseudomonas putida
6-methyl-hex-5-en-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
6-methyl-hex-5-en-2-ol + + [reduced NADPH-hemoprotein reductase] + O2
-
390112
Pseudomonas putida PpG777
6-methyl-hex-5-en-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
additional information
overview: synthetic analogues of linalool
390112
Pseudomonas putida
?
-
-
-
-
additional information
overview: synthetic analogues of linalool
390112
Pseudomonas putida PpG777
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
overview: values for cytochrome LIN P-450 with various synthetic substrates
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
both isomers of linalool, strict substrate specificity: geraniol, nerol or citronellol are not hydroxylated
390112
Pseudomonas putida
(E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390112
Pseudomonas putida
?
3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
both isomers of linalool, strict substrate specificity: geraniol, nerol or citronellol are not hydroxylated
390112
Pseudomonas putida PpG777
(E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390112
Pseudomonas putida PpG777
?
6-methyl-hex-5-en-2-ol + + [reduced NADPH-hemoprotein reductase] + O2
-
390112
Pseudomonas putida
6-methyl-hex-5-en-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
6-methyl-hex-5-en-2-ol + + [reduced NADPH-hemoprotein reductase] + O2
-
390112
Pseudomonas putida PpG777
6-methyl-hex-5-en-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
additional information
overview: synthetic analogues of linalool
390112
Pseudomonas putida
?
-
-
-
-
additional information
overview: synthetic analogues of linalool
390112
Pseudomonas putida PpG777
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
overview: values for cytochrome LIN P-450 with various synthetic substrates
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas putida
Other publictions for EC 1.14.14.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701873
Bell
A cytochrome P450 class I elec ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans ATCC 700278D-5
Appl. Microbiol. Biotechnol.
86
163-175
2010
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1
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1
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1
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1
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3
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1
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1
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1
1
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-
-
717712
Ropp
Cloning and expression of a me ...
Pseudomonas putida, Pseudomonas putida PpG777
J. Bacteriol.
175
6028-6037
1993
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1
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1
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2
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2
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1
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2
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2
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1
2
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1
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2
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1
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2
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3
3
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390114
Bernhardt
Reconstitution of cytochrome P ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
187
310-317
1992
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1
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390113
Ullah
Protein components of a cytoch ...
Pseudomonas putida, Pseudomonas putida PpG777
J. Biol. Chem.
265
1345-1351
1990
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1
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1
1
4
4
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3
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1
1
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1
2
2
13
2
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3
1
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3
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1
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3
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1
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1
1
4
4
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1
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1
2
2
13
2
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3
1
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1
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2
2
-
-
-
390112
Bhattacharyya
-
Chemical probes into the activ ...
Pseudomonas putida, Pseudomonas putida PpG777
Proc. Indian Acad. Sci. Chem. Sci.
93
1289-1304
1984
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-
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3
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6
-
1
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1
1
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6
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1
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1
1
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