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show all sequences of 1.14.14.84

A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans

Bell, S.G.; Dale, A.; Rees, N.H.; Wong, L.L.; Appl. Microbiol. Biotechnol. 86, 163-175 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha
Novosphingobium aromaticivorans
Engineering
Amino acid exchange
Commentary
Organism
additional information
interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview
Novosphingobium aromaticivorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
in the heme cofactor
Novosphingobium aromaticivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Novosphingobium aromaticivorans
-
-
-
Novosphingobium aromaticivorans ATCC 700278D-5
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography
Novosphingobium aromaticivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
NMR spectroscopy product analysis
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Novosphingobium aromaticivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Novosphingobium aromaticivorans
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Novosphingobium aromaticivorans
heme
dependent on
Novosphingobium aromaticivorans
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha
Novosphingobium aromaticivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Novosphingobium aromaticivorans
heme
dependent on
Novosphingobium aromaticivorans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview
Novosphingobium aromaticivorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
in the heme cofactor
Novosphingobium aromaticivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography
Novosphingobium aromaticivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
NMR spectroscopy product analysis
-
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Novosphingobium aromaticivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Novosphingobium aromaticivorans
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases
Novosphingobium aromaticivorans
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases
Novosphingobium aromaticivorans
Other publictions for EC 1.14.14.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701873
Bell
A cytochrome P450 class I elec ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans ATCC 700278D-5
Appl. Microbiol. Biotechnol.
86
163-175
2010
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1
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1
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717712
Ropp
Cloning and expression of a me ...
Pseudomonas putida, Pseudomonas putida PpG777
J. Bacteriol.
175
6028-6037
1993
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2
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2
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3
3
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390114
Bernhardt
Reconstitution of cytochrome P ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
187
310-317
1992
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390113
Ullah
Protein components of a cytoch ...
Pseudomonas putida, Pseudomonas putida PpG777
J. Biol. Chem.
265
1345-1351
1990
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1
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1
1
4
4
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3
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1
1
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1
2
2
13
2
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3
1
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3
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1
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3
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1
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1
1
4
4
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1
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1
2
2
13
2
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3
1
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1
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2
2
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390112
Bhattacharyya
-
Chemical probes into the activ ...
Pseudomonas putida, Pseudomonas putida PpG777
Proc. Indian Acad. Sci. Chem. Sci.
93
1289-1304
1984
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3
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6
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1
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1
1
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6
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1
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1
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