BRENDA - Enzyme Database show
show all sequences of 1.14.14.85

7-Deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures

Katano, N.; Yamamoto, H.; Iio, R.; Inoue, K.; Phytochemistry 58, 53-58 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidol
0.01 mM, 58.4% inhibition, 0.1 mM, 77.0% inhibition
Lonicera japonica
CO
60% inhibition in the dark, partially reversible by light
Lonicera japonica
cytochrome c
0.01 mM, 44.9% inhibition, 0.1 mM, 59.6% inhibition
Lonicera japonica
ketoconazole
0.01 mM, 93% inhibition, 0.1 mM, complete inhibition
Lonicera japonica
Metyrapone
0.01 mM, 73.6% inhibition, 0.1 mM, 92.4% inhibition
Lonicera japonica
miconazole
0.01 mM, 26.2% inhibition, 0.1 mM, 78.5% inhibition
Lonicera japonica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
7-deoxyloganin
-
Lonicera japonica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Lonicera japonica
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
Lonicera japonica
enzyme is involved in loganin biosynthesis
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
Lonicera japonica
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lonicera japonica
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Lonicera japonica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000099
-
activity in cell extracts, cofactor NADPH
Lonicera japonica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-deoxyloganic acid + [reduced NADPH-hemoprotein reductase] + O2
50% of activity with 7-deoxyloganin
440296
Lonicera japonica
loganic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
?
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
NADPH is essential for activity, 45% activity with NADH, substitution of O2 with N2 reduces activity by 50%
440296
Lonicera japonica
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
-
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
enzyme is involved in loganin biosynthesis
440296
Lonicera japonica
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
50% of maximal activity at pH 7.1 and pH 9.4
Lonicera japonica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidol
0.01 mM, 58.4% inhibition, 0.1 mM, 77.0% inhibition
Lonicera japonica
CO
60% inhibition in the dark, partially reversible by light
Lonicera japonica
cytochrome c
0.01 mM, 44.9% inhibition, 0.1 mM, 59.6% inhibition
Lonicera japonica
ketoconazole
0.01 mM, 93% inhibition, 0.1 mM, complete inhibition
Lonicera japonica
Metyrapone
0.01 mM, 73.6% inhibition, 0.1 mM, 92.4% inhibition
Lonicera japonica
miconazole
0.01 mM, 26.2% inhibition, 0.1 mM, 78.5% inhibition
Lonicera japonica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
7-deoxyloganin
-
Lonicera japonica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Lonicera japonica
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
Lonicera japonica
enzyme is involved in loganin biosynthesis
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
Lonicera japonica
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Lonicera japonica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000099
-
activity in cell extracts, cofactor NADPH
Lonicera japonica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-deoxyloganic acid + [reduced NADPH-hemoprotein reductase] + O2
50% of activity with 7-deoxyloganin
440296
Lonicera japonica
loganic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
?
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
NADPH is essential for activity, 45% activity with NADH, substitution of O2 with N2 reduces activity by 50%
440296
Lonicera japonica
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
-
7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2
enzyme is involved in loganin biosynthesis
440296
Lonicera japonica
loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
440296
Lonicera japonica
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
50% of maximal activity at pH 7.1 and pH 9.4
Lonicera japonica
Other publictions for EC 1.14.14.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
440296
Katano
7-Deoxyloganin 7-hydroxylase i ...
Lonicera japonica
Phytochemistry
58
53-58
2001
-
-
-
-
-
-
6
1
1
-
-
1
-
2
-
-
-
-
-
2
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
1
-
-
1
-
-
-
-
-
2
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
437786
Irmler
Indole alkaloid biosynthesis i ...
Catharanthus roseus
Plant J.
24
797-804
2000
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-