Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.86 extracted from

  • Ashman, P.J.; Mackenzie, A.; Bramley, P.M.
    Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi (1990), Biochim. Biophys. Acta, 1036, 151-157.
    View publication on PubMed
Search for more literature for 1.14.14.86

General Stability

General Stability Organism
20% glycerol reduces the degradation to P-420 Fusarium fujikuroi

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R)-paclobutrazol 0.0045 mM, 50% inhibition Fusarium fujikuroi
(2R,3R)-paclobutrazol
-
 Malus sp.
(2S,3S)-paclobutrazol 0.00025 mM, 50% inhibition Fusarium fujikuroi
(2S,3S)-paclobutrazol
-
 Malus sp.
CO 90% inhibition Fusarium fujikuroi
paclobutrazol racemate
-
 Cucurbita maxima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
 ent-kaur-16-ene
-
 Fusarium fujikuroi

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cucurbita maxima
-
-
microsome most likely membrane-associated Fusarium fujikuroi
-
-

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-
Fusarium fujikuroi
-
-
-
Fusarium fujikuroi SG78
-
-
-
Malus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Malus sp.
-
endosperm
-
 Cucurbita maxima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 2.19 Bq kaurenoids/min/mg, activity in cell extracts Fusarium fujikuroi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Cucurbita maxima ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus sp. ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi SG78 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Cucurbita maxima ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus sp. ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi SG78 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Cucurbita maxima ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Malus sp. ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fusarium fujikuroi SG78 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD stimulates activity Fusarium fujikuroi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 (2S,3S)-paclobutrazol
-
 Malus sp.
0.00002
-
 paclobutrazol racemate
-
 Cucurbita maxima
0.00006
-
 (2R,3R)-paclobutrazol
-
 Malus sp.
0.00007
-
 (2S,3S)-paclobutrazol
-
 Fusarium fujikuroi
0.0015
-
 (2R,3R)-paclobutrazol
-
 Fusarium fujikuroi