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Literature summary for 1.14.14.86 extracted from

  • Malonek, S.; Rojas, M.C.; Hedden, P.; Gaskin, P.; Hopkins, P.; Tudzynski, B.
    Functional characterization of two cytochrome P450 monooxygenase genes, P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium proliferatum (Gibberella fujikuroi MP-D) (2005), Appl. Environ. Microbiol., 71, 1462-1472.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene P450-4 from strain D02945, DNA and amino acid sequence determination and analysis, organization of the GA gene cluster, expression in Escherichia coli and in strain D02945, screening for ectopic integrations, P450-4 promoter studies using the GUS reporter system, expression analysis Fusarium proliferatum

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum Q701P2 i.e. Gibberella fujikuroi or G. intermedia, MP-C or MP-D, strains IMI58289 and D02945, gene P450-4
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 a three step oxidation Fusarium proliferatum (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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Synonyms

Synonyms Comment Organism
P450-4
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Fusarium proliferatum