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Literature summary for 1.14.14.86 extracted from

  • Humphrey, T.V.; Richman, A.S.; Menassa, R.; Brandle, J.E.
    Spatial organisation of four enzymes from Stevia rebaudiana that are involved in steviol glycoside synthesis (2006), Plant Mol. Biol., 61, 47-62.
    View publication on PubMed
Search for more literature for 1.14.14.86

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determimnation and analysis, expression in Escherichia coli, Saccharomyces cerevisiae, and in transgenic Arabidopsis thaliana leaves as GFP-tagged protein via Agrobacterium tumefaciens transfection, expression of the GFP-tagged enzyme in onion bulb epidermis or tobacco seedling leaves using particle bombardment method Stevia rebaudiana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Stevia rebaudiana 5783
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additional information subcellular compartmentation, overview Stevia rebaudiana
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Stevia rebaudiana last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
additional information Stevia rebaudiana the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Stevia rebaudiana
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-
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Stevia rebaudiana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview Stevia rebaudiana (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 a three step oxidation Stevia rebaudiana (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
additional information the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview Stevia rebaudiana ?
-
 ?

Synonyms

Synonyms Comment Organism
kaurene oxidase
-
 Stevia rebaudiana
More the enzyme belongs to the the cytochrome P450 family Stevia rebaudiana