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Literature summary for 1.14.14.86 extracted from

  • Ko, K.W.; Lin, F.; Katsumata, T.; Sugai, Y.; Miyazaki, S.; Kawaide, H.; Okada, K.; Nojiri, H.; Yamane, H.
    Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system (2008), Biosci. Biotechnol. Biochem., 72, 3285-3288.
    View publication on PubMed
Search for more literature for 1.14.14.86

Activating Compound

Activating Compound Comment Organism Structure
cytochrome P450 monooxygenase necessary for ent-kaurenoate production by ent-kaurene oxidase Oryza sativa

Application

Application Comment Organism
molecular biology the fungal cytochrome P450 monooxygenase isolated from from Phaeoshaeria sp., strain L487 in the Pichia pastoris expression system might become a tool for functional assays of a variety of rice cytochrome P450 monooxygenases involved in the biosynthesis of secondary metabolites Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification of cDNA, cloning into pPICZ yeast expression vector, expression in Pichia pastoris strain X-33 together with fungal P450 reductase (CPR) Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 Western blot analysis Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase Oryza sativa ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 30% yield of end-product ent-kaurenoate with ent-kaurenol as substrate Oryza sativa ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 6% yield of end-product ent-kaurenoate with ent-kaurene as substrate Oryza sativa ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP701A
-
 Oryza sativa
ent-kaurene oxidase
-
 Oryza sativa
OsKO2 formerly named OsKOL2, OsKO1 expression is restricted to panicles and roots Oryza sativa

General Information

General Information Comment Organism
metabolism function in the early steps of gibberellin biosynthesis Oryza sativa