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Literature summary for 1.14.14.86 extracted from

  • Morrone, D.; Chen, X.; Coates, R.M.; Peters, R.J.
    Characterization of the kaurene oxidase CYP701A3, a multifunctional cytochrome P450 from gibberellin biosynthesis (2010), Biochem. J., 431, 337-344.
    View publication on PubMed
Search for more literature for 1.14.14.86

Cloned(Commentary)

Cloned (Comment) Organism
CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
(1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
 Arabidopsis thaliana
(1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol i.e. uniconazole Arabidopsis thaliana
abscinazole-F1
-
 Arabidopsis thaliana
methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Arabidopsis thaliana the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview ?
-
 ?
additional information Arabidopsis thaliana Columbia the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93ZB2
-
-
Arabidopsis thaliana Columbia Q93ZB2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CYP701A3 partially in microsomal membranes from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview Arabidopsis thaliana ?
-
 ?
additional information the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview Arabidopsis thaliana Columbia ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP701A3
-
 Arabidopsis thaliana
kaurene oxidase
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid Arabidopsis thaliana
physiological function kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid Arabidopsis thaliana