BRENDA - Enzyme Database show
show all sequences of 1.14.14.86

The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Miyazaki, S.; Katsumata, T.; Natsume, M.; Kawaide, H.; FEBS Lett. 585, 1879-1883 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris
Physcomitrella patens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by uniconazole-P
Physcomitrella patens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
x * 62000, SDS-PAGE
Physcomitrella patens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Physcomitrella patens
-
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Physcomitrella patens
F2ZAE0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
727513
Physcomitrella patens
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 62000, SDS-PAGE
Physcomitrella patens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris
Physcomitrella patens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by uniconazole-P
Physcomitrella patens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
x * 62000, SDS-PAGE
Physcomitrella patens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Physcomitrella patens
-
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
727513
Physcomitrella patens
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 62000, SDS-PAGE
Physcomitrella patens
Other publictions for EC 1.14.14.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745244
Noguchi
Characterization of moss ent- ...
Physcomitrella patens
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CYP701A26 is characterized as ...
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Probing the single key amino ...
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Probing the promiscuity of en ...
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39
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728514
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CYP701A8: a rice ent-kaurene o ...
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2012
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744499
Todoroki
A conformationally restricted ...
Oryza sativa Japonica Group
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2012
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2
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711477
Okazaki
Abscinazole-E1, a novel chemic ...
Oryza sativa, Oryza sativa Nipponbare
Bioorg. Med. Chem.
19
406-413
2011
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726650
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Molecular cloning and identifi ...
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727513
Miyazaki
The CYP701B1 of Physcomitrella ...
Physcomitrella patens
FEBS Lett.
585
1879-1883
2011
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728510
Hamberger
Evolution of diterpene metabol ...
Picea sitchensis
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157
1677-1695
2011
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711149
Morrone
Characterization of the kauren ...
Arabidopsis thaliana, Arabidopsis thaliana Columbia
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431
337-344
2010
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1
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1
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4
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2
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688097
Sawada
Germination of photoblastic le ...
Lactuca sativa
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2008
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696834
Ko
Functional identification of a ...
Oryza sativa
Biosci. Biotechnol. Biochem.
72
3285-3288
2008
1
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676546
Humphrey
Spatial organisation of four e ...
Stevia rebaudiana
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61
47-62
2006
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660153
Swain
Plants with increased expressi ...
Arabidopsis thaliana
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46
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2005
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671411
Malonek
Functional characterization of ...
Fusarium proliferatum
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71
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2005
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676582
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The rice dwarf virus P2 protei ...
Oryza sativa, Oryza sativa Japonica Group
Plant Physiol.
139
1935-1945
2005
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2
2
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2
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12
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4
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4
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12
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660232
Davidson
The pea gene LH encodes ent-ka ...
Pisum sativum
Plant Physiol.
134
1123-1134
2004
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1
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660237
Sakamoto
An overview of gibberellin met ...
Oryza sativa
Plant Physiol.
134
1642-1653
2004
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1
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3
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746089
Itoh
A rice semi-dwarf gene, Tan-G ...
Oryza sativa Japonica Group
Plant Mol. Biol.
54
533-547
2004
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1
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440293
Helliwell
A plastid envelope location of ...
Arabidopsis thaliana
Plant J.
28
201-208
2001
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