BRENDA - Enzyme Database show
show all sequences of 1.14.14.86

Probing the single key amino acid responsible for the novel catalytic function of ent-kaurene oxidase supported by NADPH-cytochrome P450 reductases in Tripterygium wilfordii

Su, P.; Guan, H.; Zhang, Y.; Wang, X.; Gao, L.; Zhao, Y.; Hu, T.; Zhou, J.; Ma, B.; Tu, L.; Tong, Y.; Huang, L.; Gao, W.; Front. Plant Sci. 8, 1756 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant ent-kaurene oxidase expression in Saccharomyces cerevisiae strain BY-T20, coexpression with Tripterygium wilfordii CPR1-4 genes encoding four cytochrome P450 reductases, recombinant expression of enzyme mutants
Tripterygium wilfordii
Engineering
Amino acid exchange
Commentary
Organism
L387A
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387D
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16a-hydroxy-ent-kaurenoic acid
Tripterygium wilfordii
L387G
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387R
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
Tripterygium wilfordii
L387S
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387T
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
Tripterygium wilfordii
-
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
Tripterygium wilfordii
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Tripterygium wilfordii
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tripterygium wilfordii
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Tripterygium wilfordii
-
flower
-
Tripterygium wilfordii
-
leaf
-
Tripterygium wilfordii
-
additional information
transcriptome mining
Tripterygium wilfordii
-
root
-
Tripterygium wilfordii
-
stem
-
Tripterygium wilfordii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
-
744999
Tripterygium wilfordii
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744999
Tripterygium wilfordii
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744999
Tripterygium wilfordii
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
744999
Tripterygium wilfordii
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Tripterygium wilfordii
NADPH
-
Tripterygium wilfordii
Cloned(Commentary) (protein specific)
Commentary
Organism
phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant ent-kaurene oxidase expression in Saccharomyces cerevisiae strain BY-T20, coexpression with Tripterygium wilfordii CPR1-4 genes encoding four cytochrome P450 reductases, recombinant expression of enzyme mutants
Tripterygium wilfordii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Tripterygium wilfordii
NADPH
-
Tripterygium wilfordii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L387A
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387D
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16a-hydroxy-ent-kaurenoic acid
Tripterygium wilfordii
L387G
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387R
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
Tripterygium wilfordii
L387S
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
L387T
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
Tripterygium wilfordii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
Tripterygium wilfordii
-
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
Tripterygium wilfordii
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Tripterygium wilfordii
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Tripterygium wilfordii
-
flower
-
Tripterygium wilfordii
-
leaf
-
Tripterygium wilfordii
-
additional information
transcriptome mining
Tripterygium wilfordii
-
root
-
Tripterygium wilfordii
-
stem
-
Tripterygium wilfordii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
-
744999
Tripterygium wilfordii
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744999
Tripterygium wilfordii
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744999
Tripterygium wilfordii
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
744999
Tripterygium wilfordii
?
-
-
-
-
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis, gibberellin phytohormone biosynthesis, pathway overview
Tripterygium wilfordii
additional information
enzyme structur homology modeling and molecular docking, using the crystal structure of cytochrome P450 monooxygenase CYP170A1 from Streptomyces coelicolor, PDB ID 3DBG, as template. Key residue Leu387 contributes to the formation of 16alpha-hydroxy-ent-kaurenoic acid, most likely by forming hydrogen bonds with the hydroxyl group of 16alpha-hydroxy-ent-kaurane, multifunctional nature of kaurene oxidase catalysis, overview
Tripterygium wilfordii
physiological function
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid, indicating an important function of 16alpha-hydroxy-ent-kaurane in the anti-HIV principle tripterifordin biosynthetic pathway in planta
Tripterygium wilfordii
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis, gibberellin phytohormone biosynthesis, pathway overview
Tripterygium wilfordii
additional information
enzyme structur homology modeling and molecular docking, using the crystal structure of cytochrome P450 monooxygenase CYP170A1 from Streptomyces coelicolor, PDB ID 3DBG, as template. Key residue Leu387 contributes to the formation of 16alpha-hydroxy-ent-kaurenoic acid, most likely by forming hydrogen bonds with the hydroxyl group of 16alpha-hydroxy-ent-kaurane, multifunctional nature of kaurene oxidase catalysis, overview
Tripterygium wilfordii
physiological function
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid, indicating an important function of 16alpha-hydroxy-ent-kaurane in the anti-HIV principle tripterifordin biosynthetic pathway in planta
Tripterygium wilfordii
Other publictions for EC 1.14.14.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745244
Noguchi
Characterization of moss ent- ...
Physcomitrella patens
J. Biochem.
163
69-76
2018
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745584
Yamamura
Characterization of ent-kaure ...
Scoparia dulcis
J. Nat. Med.
72
456-463
2018
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744583
Mao
CYP701A26 is characterized as ...
Zea mays
Biotechnol. Lett.
39
1709-1716
2017
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11
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3
3
1
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744999
Su
Probing the single key amino ...
Tripterygium wilfordii
Front. Plant Sci.
8
1756
2017
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6
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3
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746311
Mafu
Probing the promiscuity of en ...
Arabidopsis thaliana, Oryza sativa Japonica Group
Proc. Natl. Acad. Sci. USA
113
2526-2531
2016
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2
2
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1
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8
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3
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39
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39
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746448
Su
Functional characterization o ...
Salvia miltiorrhiza
Sci. Rep.
6
23057
2016
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744175
Villa-Ruano
-
Functional characterization o ...
Montanoa tomentosa
Arch. Biol. Sci.
67
193-199
2015
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728514
Wang
CYP701A8: a rice ent-kaurene o ...
Oryza sativa
Plant Physiol.
158
1418-1425
2012
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744499
Todoroki
A conformationally restricted ...
Oryza sativa Japonica Group
Bioorg. Med. Chem. Lett.
22
3240-3243
2012
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711477
Okazaki
Abscinazole-E1, a novel chemic ...
Oryza sativa, Oryza sativa Nipponbare
Bioorg. Med. Chem.
19
406-413
2011
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726650
Lei
-
Molecular cloning and identifi ...
Momordica charantia
Afr. J. Biotechnol.
10
15724-15730
2011
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727513
Miyazaki
The CYP701B1 of Physcomitrella ...
Physcomitrella patens
FEBS Lett.
585
1879-1883
2011
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728510
Hamberger
Evolution of diterpene metabol ...
Picea sitchensis
Plant Physiol.
157
1677-1695
2011
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711149
Morrone
Characterization of the kauren ...
Arabidopsis thaliana, Arabidopsis thaliana Columbia
Biochem. J.
431
337-344
2010
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688097
Sawada
Germination of photoblastic le ...
Lactuca sativa
J. Exp. Bot.
59
3383-3393
2008
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696834
Ko
Functional identification of a ...
Oryza sativa
Biosci. Biotechnol. Biochem.
72
3285-3288
2008
1
1
1
-
-
-
-
-
-
-
1
3
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
676546
Humphrey
Spatial organisation of four e ...
Stevia rebaudiana
Plant Mol. Biol.
61
47-62
2006
-
-
1
-
-
-
-
-
2
-
-
2
-
4
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660153
Swain
Plants with increased expressi ...
Arabidopsis thaliana
Plant Cell Physiol.
46
284-291
2005
-
-
1
-
-
-
2
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671411
Malonek
Functional characterization of ...
Fusarium proliferatum
Appl. Environ. Microbiol.
71
1462-1472
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676582
Zhu
The rice dwarf virus P2 protei ...
Oryza sativa, Oryza sativa Japonica Group
Plant Physiol.
139
1935-1945
2005
-
2
2
-
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
2
2
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
3
4
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660232
Davidson
The pea gene LH encodes ent-ka ...
Pisum sativum
Plant Physiol.
134
1123-1134
2004
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660237
Sakamoto
An overview of gibberellin met ...
Oryza sativa
Plant Physiol.
134
1642-1653
2004
-
-
-
-
-
-
-
-
1
-
-
3
-
3
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746089
Itoh
A rice semi-dwarf gene, Tan-G ...
Oryza sativa Japonica Group
Plant Mol. Biol.
54
533-547
2004
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
440293
Helliwell
A plastid envelope location of ...
Arabidopsis thaliana
Plant J.
28
201-208
2001
-
-
-
-
-
-
-
-
4
-
-
-
-
5
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440294
Tudzynski
The P450-4 gene of Gibberella ...
Fusarium fujikuroi
Appl. Environ. Microbiol.
67
3514-3522
2001
-
-
1
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440292
Helliwell
Arabidopsis ent-kaurene oxidas ...
Arabidopsis thaliana
Plant Physiol.
119
507-510
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440291
Archer
Purification of ent-kaurene ox ...
Cucurbita maxima, Fusarium fujikuroi
Biochem. Soc. Trans.
20
218S
1992
-
-
-
-
-
-
-
-
2
-
1
-
-
4
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440290
Ashman
Characterization of ent-kauren ...
Cucurbita maxima, Fusarium fujikuroi, Fusarium fujikuroi SG78, Malus sp.
Biochim. Biophys. Acta
1036
151-157
1990
-
-
-
-
-
1
6
1
2
-
-
-
-
6
-
-
-
-
-
2
1
-
12
-
-
-
-
-
-
-
-
1
5
-
-
-
-
-
1
-
-
1
-
6
5
1
2
-
-
-
-
-
-
-
-
2
1
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440289
Sherwin
-
Stereospecificity of the oxida ...
Marah macrocarpa
J. Chem. Soc.
1982
1013-1014
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-