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Literature summary for 1.14.14.86 extracted from

  • Su, P.; Tong, Y.; Cheng, Q.; Hu, Y.; Zhang, M.; Yang, J.; Teng, Z.; Gao, W.; Huang, L.
    Functional characterization of ent-copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathway (2016), Sci. Rep., 6, 23057 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.86

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the enzyme from hairy roots of Salvia miltiorrhiza, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, SmKO is co-expressed with the fused enzyme SmKS-SmCPSent and a NADPH-cytochrome P450 reductase (SmCPR1) in the yeast strain SGH5 Salvia miltiorrhiza

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Salvia miltiorrhiza
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
-
 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Salvia miltiorrhiza overall reaction ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Salvia miltiorrhiza A0A0G2RKY1
-
-

Reaction

Reaction Comment Organism Reaction ID
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O overall reaction, reaction mechanism of multifunctional kaurene oxidase, overview Salvia miltiorrhiza
a

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Salvia miltiorrhiza
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Salvia miltiorrhiza ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Salvia miltiorrhiza ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
kaurene oxidase
-
 Salvia miltiorrhiza
SmKO
-
 Salvia miltiorrhiza

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Salvia miltiorrhiza
NADPH
-
 Salvia miltiorrhiza

General Information

General Information Comment Organism
metabolism the enzyme is involved in the in the gibberellin biosynthetic pathway, it catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid Salvia miltiorrhiza
physiological function in the gibberellin biosynthetic pathway. CPSent catalyzes the cyclization of geranylgeranyl diphosphate to ent-copalyl diphosphate, which is converted to ent-kaurene by kaurene synthase. Then, kaurene oxidase catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid Salvia miltiorrhiza