Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.87 extracted from

  • Sawada, Y.; Ayabe, S.
    Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue (2005), Biochem. Biophys. Res. Commun., 330, 907-913.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BJ2168 Glycyrrhiza echinata

Protein Variants

Protein Variants Comment Organism
L371V inactive Glycyrrhiza echinata
L371V/K375T the mutant shows 3-5% activity levels compared to the wild type. The mutant produces a mixture of 95% 3beta-hydroxyflavanone and 5% flavone from (2S)-liquiritigenin Glycyrrhiza echinata
S310T/K375T the P450 level of the mutant is approximately 1.5times that of wild type. The mutant shows 3-5% activity levels compared to the wild type Glycyrrhiza echinata
S310T/L371V/K375T the mutant produces 100% flavone from (2S)-liquiritigenin Glycyrrhiza echinata
S310T/L371V/K375T the mutant shows 4times higher P450 level than the wild type Glycyrrhiza echinata

Organism

Organism UniProt Comment Textmining
Glycyrrhiza echinata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-7,4'-dihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2 i.e. (2S)-liquiritigenin Glycyrrhiza echinata 2,7,4'-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
2-hydroxyisoflavanone synthase
-
Glycyrrhiza echinata
2HI synthase
-
Glycyrrhiza echinata
CYP93C
-
Glycyrrhiza echinata
IFS
-
Glycyrrhiza echinata