BRENDA - Enzyme Database show
show all sequences of 1.14.14.87

Molecular characterization of the enzyme catalyzing the aryl migration reaction of isoflavonoid biosynthesis in soybean

Steele, C.L.; Gijzen, M.; Qutob, D.; Dixon, R.A.; Arch. Biochem. Biophys. 367, 146-150 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in insect cells
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-naringenin + O2 + [reduced NADPH-hemoprotein reductase]
Glycine max
-
2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
?
liquiritigenin + O2 + [reduced NADPH-hemoprotein reductase]
Glycine max
-
2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
?
additional information
Glycine max
enzyme is more active with liquiritigenin than with naringenin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
Q9SWR5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-naringenin + O2 + [reduced NADPH-hemoprotein reductase]
-
744152
Glycine max
2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
liquiritigenin + O2 + [reduced NADPH-hemoprotein reductase]
-
744152
Glycine max
2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
additional information
enzyme is more active with liquiritigenin than with naringenin
744152
Glycine max
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 58800, calculated
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in insect cells
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-naringenin + O2 + [reduced NADPH-hemoprotein reductase]
Glycine max
-
2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
?
liquiritigenin + O2 + [reduced NADPH-hemoprotein reductase]
Glycine max
-
2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
?
additional information
Glycine max
enzyme is more active with liquiritigenin than with naringenin
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-naringenin + O2 + [reduced NADPH-hemoprotein reductase]
-
744152
Glycine max
2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
liquiritigenin + O2 + [reduced NADPH-hemoprotein reductase]
-
744152
Glycine max
2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
additional information
enzyme is more active with liquiritigenin than with naringenin
744152
Glycine max
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 58800, calculated
Glycine max
Other publictions for EC 1.14.14.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746381
Chang
Functional expression and pur ...
Medicago truncatula
Protein Expr. Purif.
150
44-52
2018
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744229
Waki
Identification of protein-pro ...
Glycine max
Biochem. Biophys. Res. Commun.
469
546-551
2016
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1
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4
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2
2
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744597
Chu
Evolutionary study of the iso ...
Glycine max
BMC Genet.
15
76
2014
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1
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1
2
2
1
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746013
Pandey
Co-expression of Arabidopsis ...
Glycine max
Plant Biotechnol. J.
12
69-80
2014
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-
1
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1
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1
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8
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1
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1
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1
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1
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1
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727120
Picmanova
-
Functional expression and subc ...
Pisum sativum
Biol. Plant.
57
635-645
2013
-
-
1
-
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-
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2
-
1
2
-
1
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1
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2
1
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1
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2
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1
2
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1
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2
1
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744040
Franzmayr
Expression and functional cha ...
Trifolium repens
Ann. Bot.
110
1291-1301
2012
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7
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1
1
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746050
Li
Metabolic engineering of isof ...
Glycine max
Plant Cell Rep.
30
1435-1442
2011
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-
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3
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1
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1
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1
1
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745537
Kim
Production of genistein from ...
Trifolium pratense
J. Microbiol. Biotechnol.
19
1612-1616
2009
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1
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716565
Lapcik
Isoflavonoids are present inA ...
no activity in Arabidopsis thaliana
Plant Physiol. Biochem.
44
106-114
2006
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6
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745464
Sreevidya
Metabolic engineering of rice ...
Glycine max
J. Exp. Bot.
57
1957-1969
2006
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4
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1
1
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671696
Sawada
Multiple mutagenesis of P450 i ...
Glycyrrhiza echinata
Biochem. Biophys. Res. Commun.
330
907-913
2005
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1
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4
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2
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4
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1
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744568
Kim
Enhancement of isoflavone syn ...
Trifolium pratense
Biotechnol. Lett.
27
1291-1294
2005
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5
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1
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1
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1
1
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746101
Deavours
Metabolic engineering of isof ...
Medicago truncatula
Plant Physiol.
138
2245-2259
2005
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5
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1
1
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746088
Dhaubhadel
Isoflavonoid biosynthesis and ...
Glycine max
Plant Mol. Biol.
53
733-743
2003
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3
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4
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660166
Sawada
Key amino acid residues requir ...
Glycyrrhiza echinata
Plant J.
31
555-564
2002
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1
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2
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3
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4
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1
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1
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711505
Akashi
New scheme of the biosynthesis ...
Glycyrrhiza echinata
Biosci. Biotechnol. Biochem.
64
2276-2279
2000
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1
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745831
Jung
Identification and expression ...
Glycine max
Nat. Biotechnol.
18
208-212
2000
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14
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6
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6
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1
2
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746139
Shimada
Induction of isoflavonoid pat ...
Lotus japonicus
Plant Sci.
160
37-47
2000
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1
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4
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744152
Steele
Molecular characterization of ...
Glycine max
Arch. Biochem. Biophys.
367
146-150
1999
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1
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714992
Hashim
Reaction mechanism of oxidativ ...
Pueraria montana var. lobata
FEBS Lett.
271
219-22
1990
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5
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1
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744827
Kochs
Enzymic synthesis of isoflavo ...
Glycine max
Eur. J. Biochem.
155
311-318
1986
1
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1
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2
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1
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