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Literature summary for 1.14.14.87 extracted from

  • Waki, T.; Yoo, D.; Fujino, N.; Mameda, R.; Denessiouk, K.; Yamashita, S.; Motohashi, R.; Akashi, T.; Aoki, T.; Ayabe, S.; Takahashi, S.; Nakayama, T.
    Identification of protein-protein interactions of isoflavonoid biosynthetic enzymes with 2-hydroxyisoflavanone synthase in soybean (Glycine max (L.) Merr.) (2016), Biochem. Biophys. Res. Commun., 469, 546-551 .
    View publication on PubMed
Search for more literature for 1.14.14.87

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Glycine max
gene ifs1, protein-protein interaction analysis using the split-ubiquitin system, in planta by BiFC, and the yeast two-hybrid system in Saccharomyces cerevisiae. No appreciable yeast growth is detected with any combinations of proteins under the assay conditions Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Glycine max 5783
-
microsome
-
 Glycine max
-
-

Organism

Organism UniProt Comment Textmining
Glycine max Q9M6B9
-
-

Synonyms

Synonyms Comment Organism
2-hydroxyisoflavanone synthase 1
-
 Glycine max
CYP93C
-
 Glycine max
GmIFS
-
 Glycine max
GmIFS1
-
 Glycine max
IFS1
-
 Glycine max

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 CYP93C Glycine max

General Information

General Information Comment Organism
metabolism (2S)-flavanones undergo 2-hydroxylation catalyzed by 2-hydroxyisoflavanone synthase (GmIFS), a microsomal cytochrome P450 enzyme (CYP93C), to produce 2-hydroxyisoflavanones pathway of isoflavone biosynthesis in soybean. Analysis of binary protein-protein interactions of 2-hydroxyisoflavanone synthase 1 (GmIFS1), a P450 (CYP93C), with cytoplasmic enzymes involved in isoflavone biosynthesis in soybean, by split-ubiquitin system. Identification of binary interactions between GmIFS1 and chalcone synthase 1 (GmCHS1), and between GmIFS1 and chalcone isomerases (GmCHIs), the interaction of GmCHS1 and GmCHIs with GmIFS1 takes place on endoplasmic reticulum on which GmIFS1 is located Glycine max
physiological function enzyme shows binary interactions with cytoplasmic chalcone synthase 1 and chalcone isomerases CHI, both in recombinant Saccharomyces cerevisiae and in planta Glycine max