BRENDA - Enzyme Database show
show all sequences of 1.14.14.87

Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity

Pandey, A.; Misra, P.; Khan, M.; Swarnkar, G.; Tewari, M.; Bhambhani, S.; Trivedi, R.; Chattopadhyay, N.; Trivedi, P.; Plant Biotechnol. J. 12, 69-80 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis
Glycine max
Engineering
Amino acid exchange
Commentary
Organism
additional information
oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols
Glycine max
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
-
2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
Q9M6D6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
-
746013
Glycine max
2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis
Glycine max
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Glycine max
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
-
2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
-
746013
Glycine max
2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Glycine max
gene ifs1, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana benthamiana leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity
up
Expression (protein specific)
Organism
Commentary
Expression
Glycine max
gene ifs1, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana benthamiana leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity
up
Other publictions for EC 1.14.14.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746381
Chang
Functional expression and pur ...
Medicago truncatula
Protein Expr. Purif.
150
44-52
2018
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744229
Waki
Identification of protein-pro ...
Glycine max
Biochem. Biophys. Res. Commun.
469
546-551
2016
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2
2
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744597
Chu
Evolutionary study of the iso ...
Glycine max
BMC Genet.
15
76
2014
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1
2
2
1
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746013
Pandey
Co-expression of Arabidopsis ...
Glycine max
Plant Biotechnol. J.
12
69-80
2014
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1
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1
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1
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8
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1
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1
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1
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727120
Picmanova
-
Functional expression and subc ...
Pisum sativum
Biol. Plant.
57
635-645
2013
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1
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2
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1
2
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1
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1
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1
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2
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1
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2
1
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744040
Franzmayr
Expression and functional cha ...
Trifolium repens
Ann. Bot.
110
1291-1301
2012
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7
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1
1
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746050
Li
Metabolic engineering of isof ...
Glycine max
Plant Cell Rep.
30
1435-1442
2011
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3
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1
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1
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1
1
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745537
Kim
Production of genistein from ...
Trifolium pratense
J. Microbiol. Biotechnol.
19
1612-1616
2009
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1
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1
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716565
Lapcik
Isoflavonoids are present in  ...
no activity in Arabidopsis thaliana
Plant Physiol. Biochem.
44
106-114
2006
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6
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745464
Sreevidya
Metabolic engineering of rice ...
Glycine max
J. Exp. Bot.
57
1957-1969
2006
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4
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1
1
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671696
Sawada
Multiple mutagenesis of P450 i ...
Glycyrrhiza echinata
Biochem. Biophys. Res. Commun.
330
907-913
2005
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1
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4
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744568
Kim
Enhancement of isoflavone syn ...
Trifolium pratense
Biotechnol. Lett.
27
1291-1294
2005
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5
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1
1
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746101
Deavours
Metabolic engineering of isof ...
Medicago truncatula
Plant Physiol.
138
2245-2259
2005
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1
1
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746088
Dhaubhadel
Isoflavonoid biosynthesis and ...
Glycine max
Plant Mol. Biol.
53
733-743
2003
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660166
Sawada
Key amino acid residues requir ...
Glycyrrhiza echinata
Plant J.
31
555-564
2002
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2
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4
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711505
Akashi
New scheme of the biosynthesis ...
Glycyrrhiza echinata
Biosci. Biotechnol. Biochem.
64
2276-2279
2000
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745831
Jung
Identification and expression ...
Glycine max
Nat. Biotechnol.
18
208-212
2000
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14
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746139
Shimada
Induction of isoflavonoid pat ...
Lotus japonicus
Plant Sci.
160
37-47
2000
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744152
Steele
Molecular characterization of ...
Glycine max
Arch. Biochem. Biophys.
367
146-150
1999
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714992
Hashim
Reaction mechanism of oxidativ ...
Pueraria montana var. lobata
FEBS Lett.
271
219-22
1990
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744827
Kochs
Enzymic synthesis of isoflavo ...
Glycine max
Eur. J. Biochem.
155
311-318
1986
1
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