BRENDA - Enzyme Database show
show all sequences of 1.14.14.88

Phenylalanine ammonia lyase functions as a switch directly controlling the accumulation of calycosin and calycosin-7-O-beta-D-glucoside in Astragalus membranaceus var. mongholicus plants

Pan, H.; Wang, Y.; Zhang, Y.; Zhou, T.; Fang, C.; Nan, P.; Wang, X.; Li, X.; Wei, Y.; Chen, J.; J. Exp. Bot. 59, 3027-3037 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
Astragalus membranaceus var. mongholicus is a medicinal plant in traditional Chinese medicine, the enzyme products calycosin and calycosin-7-O-beta-D-glucoside are two major isoflavones related to the bioactivity of the herb
Astragalus mongholicus
Cloned(Commentary)
Commentary
Organism
PCR-amplification
Astragalus mongholicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Astragalus mongholicus
-
calycosin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Astragalus mongholicus
Q2ENF7
var. mongholicus
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Astragalus mongholicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
693416
Astragalus mongholicus
calycosin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
medicine
Astragalus membranaceus var. mongholicus is a medicinal plant in traditional Chinese medicine, the enzyme products calycosin and calycosin-7-O-beta-D-glucoside are two major isoflavones related to the bioactivity of the herb
Astragalus mongholicus
Cloned(Commentary) (protein specific)
Commentary
Organism
PCR-amplification
Astragalus mongholicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Astragalus mongholicus
-
calycosin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Astragalus mongholicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
693416
Astragalus mongholicus
calycosin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Astragalus mongholicus
gradual increase (1.8fold) of enzyme expression after low-temperature treatment (4 h 2C) of plants adapted to 16C, dramatic increase of expression in warm-treatment plants (16C to 25C or from 2C to 25C), maximum at h 4 during treatment, higher levels than at low-temperature induction, cold-induced mRNA accumulation is light dependent
up
General Information
General Information
Commentary
Organism
physiological function
calycosin-7-O-beta-D-glucoside pathway, calycosin and calycosin-7-O-beta-D-glucoside accumulate in whole plants, mainly leaves, in response to low temperature
Astragalus mongholicus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
calycosin-7-O-beta-D-glucoside pathway, calycosin and calycosin-7-O-beta-D-glucoside accumulate in whole plants, mainly leaves, in response to low temperature
Astragalus mongholicus
Expression (protein specific)
Organism
Commentary
Expression
Astragalus mongholicus
gradual increase (1.8fold) of enzyme expression after low-temperature treatment (4 h 2C) of plants adapted to 16C, dramatic increase of expression in warm-treatment plants (16C to 25C or from 2C to 25C), maximum at h 4 during treatment, higher levels than at low-temperature induction, cold-induced mRNA accumulation is light dependent
up
Other publictions for EC 1.14.14.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
693416
Pan
Phenylalanine ammonia lyase fu ...
Astragalus mongholicus
J. Exp. Bot.
59
3027-3037
2008
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1
1
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3
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1
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1
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1
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1
1
1
1
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-
689509
Pan
Accumulation of calycosin and ...
Astragalus mongholicus
Plant Cell Rep.
26
1111-1120
2007
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-
1
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1
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2
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5
1
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1
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1
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5
1
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2
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660173
Liu
Regiospecific hydroxylation of ...
Medicago truncatula
Plant J.
36
471-484
2003
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-
-
-
-
-
-
2
1
-
-
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1
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2
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4
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2
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1
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2
1
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2
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4
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2
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1
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285320
Clemens
-
Characterization of cytochrome ...
Cicer arietinum
Phytochemistry
32
653-657
1993
-
-
-
-
-
-
6
2
-
-
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1
-
1
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-
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3
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1
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1
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6
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2
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1
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3
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1
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1
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285319
Daniel
Elicitor-induced metabolic cha ...
Cicer arietinum
Planta
182
270-278
1990
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1
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2
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1
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285318
Hinderer
-
Microsomal isoflavone 2'- and ...
Cicer arietinum
FEBS Lett.
214
101-106
1987
2
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2
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1
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1
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1
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1
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5
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5
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