BRENDA - Enzyme Database show
show all sequences of 1.14.14.89

Microsomal isoflavone 2'- and 3'-hydroxylases from chickpea (Cicer arietinum L.) cell suspensions induced for pterocarpan phytoalexin formation

Hinderer, W.; Flentje, U.; Barz, W.; FEBS Lett. 214, 101-106 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity
Cicer arietinum
NADH
135% of NADPH activity; synergistic effect with NADPH
Cicer arietinum
Inhibitors
Inhibitors
Commentary
Organism
Structure
cytochrome c
0.01 mM cytochrome c reduces activity to 14%, 0.1 mM cytochrome c reduces activity to 4%
Cicer arietinum
additional information
no inhibition by 5 mM KCN and 1 mM EDTA
Cicer arietinum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Cicer arietinum
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Cicer arietinum
involved in the biosynthesis of the pterocarpin phytoalexin medicarpin
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Cicer arietinum
cytochrome P450-dependent monooxygenase
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cicer arietinum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
suspension
Cicer arietinum
-
cell suspension culture
-
Cicer arietinum
-
Storage Stability
Storage Stability
Organism
-20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h
Cicer arietinum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
285318
Cicer arietinum
ir
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2.0% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
involved in the biosynthesis of the pterocarpin phytoalexin medicarpin
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
cytochrome P450-dependent monooxygenase
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
78.1% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
2.2% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein
285318
Cicer arietinum
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay conditions
Cicer arietinum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors are FAD and FMN
Cicer arietinum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity
Cicer arietinum
NADH
135% of NADPH activity; synergistic effect with NADPH
Cicer arietinum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors are FAD and FMN
Cicer arietinum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cytochrome c
0.01 mM cytochrome c reduces activity to 14%, 0.1 mM cytochrome c reduces activity to 4%
Cicer arietinum
additional information
no inhibition by 5 mM KCN and 1 mM EDTA
Cicer arietinum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Cicer arietinum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Cicer arietinum
involved in the biosynthesis of the pterocarpin phytoalexin medicarpin
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Cicer arietinum
cytochrome P450-dependent monooxygenase
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
suspension
Cicer arietinum
-
cell suspension culture
-
Cicer arietinum
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h
Cicer arietinum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
285318
Cicer arietinum
ir
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2.0% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
involved in the biosynthesis of the pterocarpin phytoalexin medicarpin
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
cytochrome P450-dependent monooxygenase
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
78.1% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
2.2% of the activity with biochanin A
285318
Cicer arietinum
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein
285318
Cicer arietinum
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay conditions
Cicer arietinum
Other publictions for EC 1.14.14.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745660
Gao
Reduction of soy isoflavones ...
Slackia sp. AUH-JLC159
Lett. Appl. Microbiol.
63
111-116
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
3
-
1
-
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-
1
-
-
2
-
-
-
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-
1
2
-
1
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-
-
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2
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-
-
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-
-
-
-
3
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
285324
Shimada
Induction of isoflavonoid path ...
Lotus japonicus
Plant Sci.
160
37-47
2000
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-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
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-
1
-
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1
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-
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
285323
Akashi
CYP81E1, a cytochrome P450 cDN ...
Glycyrrhiza echinata
Biochem. Biophys. Res. Commun.
251
67-70
1998
-
-
1
-
-
-
-
-
1
-
-
1
-
1
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1
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5
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1
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1
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1
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1
-
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5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285320
Clemens
-
Characterization of cytochrome ...
Cicer arietinum
Phytochemistry
32
653-657
1993
-
-
-
-
-
-
7
2
-
1
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
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-
-
-
7
-
2
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285319
Daniel
Elicitor-induced metabolic cha ...
Cicer arietinum
Planta
182
270-278
1990
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
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-
1
-
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2
-
1
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1
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1
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1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285318
Hinderer
-
Microsomal isoflavone 2'- and ...
Cicer arietinum
FEBS Lett.
214
101-106
1987
2
-
-
-
-
-
2
-
1
-
-
2
-
1
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285325
Kochs
Enzymic synthesis of isoflavon ...
Glycine max
Eur. J. Biochem.
155
311-318
1986
1
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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