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Literature summary for 1.14.14.9 extracted from

  • Blakley, E.R.
    The catabolism of L-tyrosine by an Arthrobacter sp. (1977), Can. J. Microbiol., 23, 1128-1139.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits non-purified enzyme, ammonium sulfate precipitate Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 Arthrobacter sp. degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 Arthrobacter sp. PRL W15 degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
PRL W15
-
Arthrobacter sp. PRL W15
-
PRL W15
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation, ammonium sulfate precipitation Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity is reduced by about 40% if 3-hydroxyphenylacetate is used as substrate instead of 4-hydroxyphenylacetate Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyphenylacetate + NAD(P)H + O2
-
Arthrobacter sp. 3,4-hydroxyphenylacetate + NAD(P)+ + H2O
-
?
3-hydroxyphenylacetate + NAD(P)H + O2
-
Arthrobacter sp. PRL W15 3,4-hydroxyphenylacetate + NAD(P)+ + H2O
-
?
4-hydroxyphenylacetate + NAD(P)H + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Arthrobacter sp. 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
ir
4-hydroxyphenylacetate + NAD(P)H + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Arthrobacter sp. PRL W15 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
ir
4-hydroxyphenylacetate + NADH + O2 degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate Arthrobacter sp. 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate Arthrobacter sp. PRL W15 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
additional information similar enzyme which oxidizes both NADH and NADPH Arthrobacter sp.