Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits non-purified enzyme, ammonium sulfate precipitate | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Arthrobacter sp. | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | Arthrobacter sp. PRL W15 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
PRL W15 | - |
Arthrobacter sp. PRL W15 | - |
PRL W15 | - |
Purification (Comment) | Organism |
---|---|
ultracentrifugation, ammonium sulfate precipitation | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity is reduced by about 40% if 3-hydroxyphenylacetate is used as substrate instead of 4-hydroxyphenylacetate | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Arthrobacter sp. | 3,4-hydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Arthrobacter sp. PRL W15 | 3,4-hydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NAD(P)H + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Arthrobacter sp. | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
ir | |
4-hydroxyphenylacetate + NAD(P)H + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Arthrobacter sp. PRL W15 | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
ir | |
4-hydroxyphenylacetate + NADH + O2 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | Arthrobacter sp. | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | Arthrobacter sp. PRL W15 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | similar enzyme which oxidizes both NADH and NADPH | Arthrobacter sp. |