KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-steady kinetics, kinetics of FMN reduction, transfer from C1 to C2 component, and FMNH- release, thermodynamic data, including redox potentials, and Kd values, detailed overview | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + H+ + O2 | Acinetobacter baumannii | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
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Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | kinetic mechanism of enzyme components C1 and C2 | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + H+ + O2 | - |
Acinetobacter baumannii | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + H+ + O2 | HPAH is composed of two proteins: a FMN reductase C1 and an oxygenase C2, C1 catalyzes the reduction of FMN by NADH to generate reduced FMN, FMNH2, for use by C2 in the hydroxylation reaction, C1 is unique among the flavin reductases in that the substrate 4-hydroxyphenylacetate stimulates the rates of both the reduction of FMN and release of FMNH2 from the enzyme, the dissociation of FMNH2 from C1 is rate-limiting in the intermolecular transfer of FMNH2 from C1 to C2, and this process is regulated by the presence of 4-hydroxyphenylacetate , overview | Acinetobacter baumannii | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
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Subunits | Comment | Organism |
---|---|---|
More | HPAH is composed of two proteins: a FMN reductase C1 and an oxygenase C2 | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
HPAH | - |
Acinetobacter baumannii |
p-hydroxyphenylacetate hydroxylase | - |
Acinetobacter baumannii |
two-component p-hydroxyphenylacetate hydroxylase | - |
Acinetobacter baumannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter baumannii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | utilized by C1 | Acinetobacter baumannii | |
FMNH2 | utiluzed by C2 | Acinetobacter baumannii | |
NADH | - |
Acinetobacter baumannii |