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Literature summary for 1.14.14.91 extracted from

  • Gravot, A.; Larbat, R.; Hehn, A.; Lievre, K.; Gontier, E.; Goergen, J.L.; Bourgaud, F.
    Cinnamic acid 4-hydroxylase mechanism-based inactivation by psoralen derivatives: cloning and characterization of a C4H from a psoralen producing plant-Ruta graveolens-exhibiting low sensitivity to psoralen inactivation (2004), Arch. Biochem. Biophys., 422, 71-80.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Ruta graveolens
expression in Saccharomyces cerevisiae Petroselinum crispum
expression in Saccharomyces cerevisiae Helianthus tuberosus

Inhibitors

Inhibitors Comment Organism Structure
8-methoxypsoralen
-
 Helianthus tuberosus
8-methoxypsoralen
-
 Petroselinum crispum
8-methoxypsoralen
-
 Ruta graveolens
psoralen
-
 Helianthus tuberosus
psoralen
-
 Petroselinum crispum
psoralen irreversibly bound to the apoprotein. Inactivation is dependent on the presence of NADPH, time of pre-incubation, and inhibitor concentration Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 trans-cinnamate pH 7.4 Ruta graveolens
1
-
 trans-cinnamate pH 7.4 Helianthus tuberosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 Petroselinum crispum key step in biosynthesis of phenylpropanoic compounds 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Helianthus tuberosus Q04468
-
-
Petroselinum crispum Q43033
-
-
Ruta graveolens Q9AR74
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Ruta graveolens
-
leaf
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Ruta graveolens 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Petroselinum crispum 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Helianthus tuberosus 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 key step in biosynthesis of phenylpropanoic compounds Petroselinum crispum 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cinnamic acid 4-hydroxylase
-
 Ruta graveolens
cinnamic acid 4-hydroxylase
-
 Petroselinum crispum
cinnamic acid 4-hydroxylase
-
 Helianthus tuberosus
CYP73A1
-
 Helianthus tuberosus
CYP73A10
-
 Petroselinum crispum
CYP73A32
-
 Ruta graveolens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
129.1
-
 trans-cinnamate pH 7.4 Helianthus tuberosus
161.8
-
 trans-cinnamate pH 7.4 Ruta graveolens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0048
-
 8-methoxypsoralen pH 7.4 Helianthus tuberosus
0.0054
-
 psoralen pH 7.4 Helianthus tuberosus
0.0077
-
 psoralen pH 7.4 Ruta graveolens
0.00812
-
 psoralen pH 7.4 Petroselinum crispum
0.0142
-
 8-methoxypsoralen pH 7.4 Petroselinum crispum
0.0326
-
 8-methoxypsoralen pH 7.4 Ruta graveolens