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Literature summary for 1.14.14.92 extracted from

  • Fukuda, H.; Nakamura, K.; Sukita, E.; Ogawa, T.; Fujii, T.
    Cytochrome P450rm from Rhodotorula minuta catalyzes 4-hydroxylation of benzoate (1996), J. Biochem., 119, 314-318.
    View publication on PubMed
Search for more literature for 1.14.14.92

Inhibitors

Inhibitors Comment Organism Structure
CO strong inhibition Cystobasidium minutum
m-Hydroxybenzoate
-
 Cystobasidium minutum
salicylate
-
 Cystobasidium minutum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome more than 90% of activity Cystobasidium minutum
-
-

Organism

Organism UniProt Comment Textmining
Cystobasidium minutum
-
 red yeast
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Cystobasidium minutum 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cinnamate + [reduced NADPH-hemoprotein reductase] + O2 very low turnover rate Cystobasidium minutum 4-coumarate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 cinnamate reconstituted enzyme system consisting of benzoate 4-hydroxylase i.e. P450rm, cytochrome P450 reductase, NADPH and dilauroylphosphatidylcholine Cystobasidium minutum
0.667
-
 benzoate reconstituted enzyme system consisting of benzoate 4-hydroxylase i.e. P450rm, cytochrome P450 reductase, NADPH and dilauroylphosphatidylcholine Cystobasidium minutum
1
-
 benzoate turnover rate in microsomes Cystobasidium minutum

Cofactor

Cofactor Comment Organism Structure
additional information tetrahydropteridine has no effect on benzoate 4-hydroxylase activity Cystobasidium minutum