BRENDA - Enzyme Database show
show all sequences of 1.14.14.92

Molecular characterization of cytochrome P450 catalyzing hydroxylation of benzoates from the white-rot fungus Phanerochaete chrysosporium

Matsuzaki, F.; Wariishi, H.; Biochem. Biophys. Res. Commun. 334, 1184-1190 (2005)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
benzoic acid induces transcription of the gene encoding the enzyme and has a regulatory effect
Phanerochaete chrysosporium
Cloned(Commentary)
Commentary
Organism
gene CYP1f, DNA and amino acid sequence determination and analysis, five introns and six exons, functional expression in Pichia pastoris
Phanerochaete chrysosporium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Phanerochaete chrysosporium
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
heme iron, the heme-binding domain FSFGPRSCVG at residues 421–430 is located in the C-terminal region
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, about, amino acid sequence calculation
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Phanerochaete chrysosporium
benzoic acid has a regulatory effect on the enzyme
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
Q4H2B4
white-rot fungus, a lignin-degrading basidiomycete, ATCC 34541
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylbenzoic acid + NADPH + O2
-
671704
Phanerochaete chrysosporium
4-hydroxy-2-methylbenzoic acid + NADP+ + H2O
-
-
-
?
3-hydroxybenzoic acid + [reduced NADPH-hemoprotein reductase] + O2
reaction of 3-hydroxybenzoate 4-monooxygenase, EC 1.14.13.23
671704
Phanerochaete chrysosporium
3,4-dihydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-methylbenzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671704
Phanerochaete chrysosporium
4-hydroxy-3-methylbenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671704
Phanerochaete chrysosporium
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
benzoic acid has a regulatory effect on the enzyme
671704
Phanerochaete chrysosporium
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, about, amino acid sequence calculation
Phanerochaete chrysosporium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Phanerochaete chrysosporium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
benzoic acid induces transcription of the gene encoding the enzyme and has a regulatory effect
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP1f, DNA and amino acid sequence determination and analysis, five introns and six exons, functional expression in Pichia pastoris
Phanerochaete chrysosporium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Phanerochaete chrysosporium
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
heme iron, the heme-binding domain FSFGPRSCVG at residues 421–430 is located in the C-terminal region
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, about, amino acid sequence calculation
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
Phanerochaete chrysosporium
benzoic acid has a regulatory effect on the enzyme
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylbenzoic acid + NADPH + O2
-
671704
Phanerochaete chrysosporium
4-hydroxy-2-methylbenzoic acid + NADP+ + H2O
-
-
-
?
3-hydroxybenzoic acid + [reduced NADPH-hemoprotein reductase] + O2
reaction of 3-hydroxybenzoate 4-monooxygenase, EC 1.14.13.23
671704
Phanerochaete chrysosporium
3,4-dihydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-methylbenzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671704
Phanerochaete chrysosporium
4-hydroxy-3-methylbenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
671704
Phanerochaete chrysosporium
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
benzoic acid has a regulatory effect on the enzyme
671704
Phanerochaete chrysosporium
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, about, amino acid sequence calculation
Phanerochaete chrysosporium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Phanerochaete chrysosporium
Other publictions for EC 1.14.14.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741716
Antunes
A benzoate-activated promoter ...
Aspergillus niger
Appl. Microbiol. Biotechnol.
100
5479-5489
2016
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1
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1
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1
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-
742764
Korosec
Antifungal activity of cinnam ...
Curvularia lunata
J. Appl. Microbiol.
116
955-966
2014
-
2
1
-
-
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1
-
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5
-
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1
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2
1
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1
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1
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-
-
-
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-
2
2
-
-
-
725603
Berne
Virtual screening yields inhib ...
Curvularia lunata
J. Chem. Inf. Model.
52
3053-3063
2012
-
-
-
1
-
-
2
-
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-
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-
2
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2
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1
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2
2
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686803
Kim
Molecular cloning and function ...
Chromohalobacter sp. HS2
FEMS Microbiol. Lett.
280
235-241
2008
-
-
1
-
-
-
-
-
-
1
-
1
-
3
-
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1
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1
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1
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1
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1
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-
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-
671533
Shiningavamwe
Heterologous expression of the ...
Cystobasidium minutum, Cystobasidium minutum CBS 2177
Appl. Microbiol. Biotechnol.
72
323-329
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
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-
-
1
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2
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1
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2
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1
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2
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-
671704
Matsuzaki
Molecular characterization of ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
334
1184-1190
2005
1
-
1
-
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1
1
1
1
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1
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5
1
1
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1
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1
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1
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1
1
1
1
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5
1
1
-
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-
1
-
-
-
-
-
-
-
-
-
440261
Faber
Purification and characterizat ...
Aspergillus niger
Arch. Biochem. Biophys.
394
245-254
2001
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
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1
-
-
-
-
12
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
440259
Fukuda
Cytochrome P450rm from Rhodoto ...
Cystobasidium minutum
J. Biochem.
119
314-318
1996
-
-
-
-
-
-
3
-
1
-
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-
4
-
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2
-
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-
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3
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1
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1
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3
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1
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-
2
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3
-
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-
-
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-
440260
Van Den Brink
Optimization of the benzoate-i ...
Aspergillus niger, Aspergillus niger N204
Appl. Microbiol. Biotechnol.
46
360-364
1996
-
-
1
-
-
-
-
-
-
-
-
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-
2
-
-
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-
-
-
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2
-
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1
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1
-
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-
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-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
440254
McNamee
Properties of a membrane-assoc ...
Rhodotorula graminis
Biochem. Biophys. Res. Commun.
129
485-492
1985
-
-
1
-
-
-
15
2
1
-
-
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-
3
-
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-
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-
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1
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1
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2
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1
2
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15
-
2
1
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1
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1
-
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-
-
-
-
4804
Durham
Initial reactions involved in ...
Rhodotorula graminis
J. Bacteriol.
160
778-780
1984
-
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1
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1
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1
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-
440258
Shailubhai
-
Degradation of chlorinated der ...
Aspergillus niger
FEMS Microbiol. Lett.
18
279-282
1983
-
-
-
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1
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1
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1
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1
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1
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440255
Reddy
Purification and properties of ...
Pseudomonas sp.
Arch. Biochem. Biophys.
177
488-498
1976
3
-
-
-
-
2
9
3
-
1
1
-
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1
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1
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1
2
3
-
1
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1
1
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1
1
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3
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1
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2
-
9
1
3
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1
1
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1
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1
2
3
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1
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1
1
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-
440256
Reddy
Purification, properties and i ...
Aspergillus niger, Aspergillus niger UBC 814
Biochim. Biophys. Acta
384
46-57
1975
1
-
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3
13
2
-
1
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6
-
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1
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2
1
1
2
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1
1
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1
1
1
1
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1
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1
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3
-
13
-
2
-
1
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1
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2
1
1
2
-
1
1
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1
1
1
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