BRENDA - Enzyme Database show
show all sequences of 1.14.14.93

Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures

Hagmann, M.L.; Heller, W.; Grisebach, H.; Eur. J. Biochem. 142, 127-131 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cytochrome c
-
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
increases activity
Glycine max
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
Glycine max
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycine max
-
seedling
-
Glycine max
-
Storage Stability
Storage Stability
Organism
-70°C, storage of microsomes, 50% loss of activity in 3-4 weeks
Glycine max
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
390057
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390057
Glycine max
?
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
390057
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390057
Glycine max
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Glycine max
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
-
75% of activity maximum at 30°C
Glycine max
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half-life: 9 min, in 0.08 M potassium phosphate buffer, pH 7.5, 14 mM 2-mercaptoethanol, 20% sucrose, crude enzyme extract
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
8.2
in Tris/HCl and potassium phosphate
Glycine max
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
possibly a heme-thiolate protein
Glycine max
FAD
at 0.05 mM with 0.05 mM FMN and 0.01 mM NADPH: 132% relative activity to NADPH alone
Glycine max
FMN
at 0.05 mM with 0.05 mM FAD and 0.01 mM NADPH: 132% relative activity to NADPH alone
Glycine max
additional information
with NADH, ascorbic acid, FAD, FMN or 6,7-dimethyl-5,6,7,8-tetrahydropterine alone no activity can be detected
Glycine max
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
possibly a heme-thiolate protein
Glycine max
FAD
at 0.05 mM with 0.05 mM FMN and 0.01 mM NADPH: 132% relative activity to NADPH alone
Glycine max
FMN
at 0.05 mM with 0.05 mM FAD and 0.01 mM NADPH: 132% relative activity to NADPH alone
Glycine max
additional information
with NADH, ascorbic acid, FAD, FMN or 6,7-dimethyl-5,6,7,8-tetrahydropterine alone no activity can be detected
Glycine max
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cytochrome c
-
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
increases activity
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
Glycine max
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycine max
-
seedling
-
Glycine max
-
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, storage of microsomes, 50% loss of activity in 3-4 weeks
Glycine max
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
390057
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390057
Glycine max
?
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
390057
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390057
Glycine max
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Glycine max
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
-
75% of activity maximum at 30°C
Glycine max
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half-life: 9 min, in 0.08 M potassium phosphate buffer, pH 7.5, 14 mM 2-mercaptoethanol, 20% sucrose, crude enzyme extract
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
8.2
in Tris/HCl and potassium phosphate
Glycine max
Other publictions for EC 1.14.14.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390062
Schopfer
Molecular characterization and ...
Glycine max
FEBS Lett.
432
182-186
1998
-
-
1
-
-
-
-
2
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
348370
Kochs
Further characterization of cy ...
Glycine max
Arch. Biochem. Biophys.
293
187-194
1992
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390061
Benveniste
Multiple forms of NADPH-cytoch ...
Glycine max
Biochem. Biophys. Res. Commun.
177
105-112
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390058
Kochs
Phytoalexin synthesis in soybe ...
Glycine max
Arch. Biochem. Biophys.
273
543-553
1989
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390060
Bonhoff
Race: Cultivar-specific induct ...
Glycine max
Arch. Biochem. Biophys.
246
149-154
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390057
Hagmann
Induction of phytoalexin synth ...
Glycine max
Eur. J. Biochem.
142
127-131
1984
-
-
-
-
-
-
1
-
1
1
-
1
-
2
-
-
-
-
-
2
-
1
2
-
1
1
1
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-